The VGF-derived peptide TLQP-21: A new modulatory peptide for inflammatory pain

Roberto Rizzi, Alessandro Bartolomucci, Anna Moles, Francesca D'Amato, Paola Sacerdote, Andrea Levi, Giorgio La Corte, Maria Teresa Ciotti, Roberta Possenti, Flaminia Pavone

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39 Scopus citations


Vgf, is a neuro-endocrine specific gene encoding for a large protein precursor of different peptides. A role for VGF in pain modulation has been suggested from immunohistochemical studies showing VGF mRNA widely expressed in primary sensory neurons. In this study, the presence of VGF on the primary sensory afferents in mice was confirmed by showing its immunostaining in cultured neurons of dorsal root ganglia in secretory granule varicosities colocalized with Substance P. Moreover, the functional role of a C-terminal internal VGF-derived peptide, i.e. TLQP-21, was assessed by investigating its peripheral (1, 2, 4, 8 mM) and central (1, 2, 4 mM) effects on inflammatory pain in the formalin test. A significant increase of pain-related licking response following peripheral injection of TLQP-21 (4 and 8 mM) was observed in the second inflammatory phase of the test. In addition, an increase in licking response was detected when 4 mM of the peptide was injected alone without formalin. On the other hand, the central administration of TLQP-21 induced an U-shaped curve, with the dose of 2 mM being analgesic during the second phase. This study shows for the first time that a VGF-derived peptide may be involved in inflammatory pain in vivo and demonstrates a different action for TLQP21 at the peripheral and central levels of the nociceptive pathways.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalNeuroscience Letters
Issue number1
StatePublished - Aug 15 2008
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful to Dr. Fulvio Florenzano for his precious support with confocal microscopy. This study was supported by FIRB RBNE01JKLF_ 004 to AM, FIRB RBNE01JKLF_006 and FIRB RBNE013XSJ_004 to RP, and FISR Neurobiotecnologie and Telethon E0830 to AL.

Copyright 2008 Elsevier B.V., All rights reserved.


  • Behaviour
  • DRG
  • Formalin pain
  • Immunofluorescence
  • Mice
  • Substance P


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