The utility of diagnostic tests for immune-mediated hemolytic anemia

Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task Force

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Background: A definitive diagnosis of immune-mediated hemolytic anemia (IMHA) can be difficult to make. However, it is critical to differentiate IMHA from other causes of anemia due to the impact on prognosis and outcome for IMHA patients. Recently published American College of Veterinary Internal Medicine recommendations for the diagnosis of IMHA should be followed to concurrently confirm ongoing anemia, verify in vivo hemolysis, and detect anti-erythrocyte antibodies. The reliability of immunologic IMHA tests varies depending on which test is used and how it is performed. Objectives: Our aims were to determine which tests are currently used in veterinary medicine to diagnose IMHA and review the utility of assays that have historically been used to diagnose IMHA. Methods: A short survey was designed to see which diagnostic tests for IMHA were currently being used by veterinary practices. The survey was distributed via list-serves to veterinarians and veterinary technologists. A literature review was performed to report the utility of diagnostic tests for the diagnosis of IMHA. Results: Survey respondents indicated a variability in test protocols used to diagnose IMHA. Most respondents perform saline agglutination or Coombs’ tests to detect anti-erythrocyte antibodies. Additional tests that can be used to support a diagnosis of IMHA are discussed in this review. Conclusions: A standardized diagnostic approach should be followed to differentiate IMHA from other causes of anemia. Test methodology can vary from one laboratory to another, and clinicians should be familiar with the procedures used by their laboratory.

Original languageEnglish (US)
Pages (from-to)7-16
Number of pages10
JournalVeterinary Clinical Pathology
Volume48
Issue numberS1
DOIs
StatePublished - Oct 1 2019

Fingerprint

hemolytic anemia
Hemolytic Anemia
Routine Diagnostic Tests
diagnostic techniques
anemia
Anemia
testing
Veterinary Medicine
Anti-Idiotypic Antibodies
erythrocytes
Erythrocytes
Coombs Test
Agglutination Tests
antibodies
Veterinarians
hemolysis
agglutination
Internal Medicine
Hemolysis
veterinary medicine

Keywords

  • Coombs’
  • agglutination
  • anti-erythrocyte antibody
  • diagnosis
  • spherocytosis

PubMed: MeSH publication types

  • Journal Article
  • Review

Cite this

Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task Force (2019). The utility of diagnostic tests for immune-mediated hemolytic anemia. Veterinary Clinical Pathology, 48(S1), 7-16. https://doi.org/10.1111/vcp.12771

The utility of diagnostic tests for immune-mediated hemolytic anemia. / Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task Force.

In: Veterinary Clinical Pathology, Vol. 48, No. S1, 01.10.2019, p. 7-16.

Research output: Contribution to journalReview article

Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task Force 2019, 'The utility of diagnostic tests for immune-mediated hemolytic anemia', Veterinary Clinical Pathology, vol. 48, no. S1, pp. 7-16. https://doi.org/10.1111/vcp.12771
Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task Force. The utility of diagnostic tests for immune-mediated hemolytic anemia. Veterinary Clinical Pathology. 2019 Oct 1;48(S1):7-16. https://doi.org/10.1111/vcp.12771
Members of the Veterinary and Comparative Clinical Immunology Society Diagnostic Task Force. / The utility of diagnostic tests for immune-mediated hemolytic anemia. In: Veterinary Clinical Pathology. 2019 ; Vol. 48, No. S1. pp. 7-16.
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