The use of three-dimensional nanostructures to instruct cells to produce extracellular matrix for regenerative medicine strategies

Katja Schenke-Layland, Fady Rofail, Sanaz Heydarkhan, Jessica M. Gluck, Nilesh P. Ingle, Ekaterini Angelis, Chang Hwan Choi, William R. MacLellan, Ramin E. Beygui, Richard J. Shemin, Sepideh Heydarkhan-Hagvall

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Synthetic polymers or naturally-derived extracellular matrix (ECM) proteins have been used to create tissue engineering scaffolds; however, the need for surface modification in order to achieve polymer biocompatibility and the lack of biomechanical strength of constructs built using proteins alone remain major limitations. To overcome these obstacles, we developed novel hybrid constructs composed of both strong biosynthetic materials and natural human ECM proteins. Taking advantage of the ability of cells to produce their own ECM, human foreskin fibroblasts were grown on silicon-based nanostructures exhibiting various surface topographies that significantly enhanced ECM protein production. After 4 weeks, cell-derived sheets were harvested and histology, immunochemistry, biochemistry and multiphoton imaging revealed the presence of collagens, tropoelastin, fibronectin and glycosaminoglycans. Following decellularization, purified sheet-derived ECM proteins were mixed with poly(ε-caprolactone) to create fibrous scaffolds using electrospinning. These hybrid scaffolds exhibited excellent biomechanical properties with fiber and pore sizes that allowed attachment and migration of adipose tissue-derived stem cells. Our study represents an innovative approach to generate strong, non-cytotoxic scaffolds that could have broad applications in tissue regeneration strategies.

Original languageEnglish (US)
Pages (from-to)4665-4675
Number of pages11
JournalBiomaterials
Volume30
Issue number27
DOIs
StatePublished - Sep 1 2009

Keywords

  • Biomimetic material
  • ECM
  • Electrospinning
  • Multiphoton imaging
  • Nanotopography
  • Tissue engineering

Fingerprint Dive into the research topics of 'The use of three-dimensional nanostructures to instruct cells to produce extracellular matrix for regenerative medicine strategies'. Together they form a unique fingerprint.

Cite this