Abstract
A procedure is described to quantitate inorganic phosphate in the form of the tris(trimethylsilyl) (TMS) phosphate by gas-liquid chromatography-mass spectrometry (glems) that increases the previously reported detection limit from the microgram to the nanogram range (i.e., 3 ng). The sensitivity for detecting TMS-phosphate by glc-ms analysis was shown to be limited by an increasing fractional loss with decreasing concentrations of TMS-phosphate analyzed due to its adsorption on different types of glc column supports. The method developed employs [18O4]phosphoric acid which serves as both an internal standard to permit quantitation and as a carrier to minimize sample adsorption on the glc column support.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 324-331 |
| Number of pages | 8 |
| Journal | Analytical Biochemistry |
| Volume | 107 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1980 |
Bibliographical note
Funding Information:We would like to extend our gratitude to the University of Minnesota Agricultural Experiment Station and to Dr. R. T. Holman and Mr. W. Heimer-man (University of Minnesota, the Hormal Institute) for providing the glc-ms facilities. This research was supported by grants from U. S. Public Health Service, NS 05979; American Cancer Society, BC-166: and the Minnesota Leukemia Research Fund.