The use of [18O4]phosphoric acid in the quantitation of phosphate by gas-liquid chromatography-mass spectrometry analysis

Gustav Graff, Thomas P. Krick, Timothy F. Walseth, Nelson D. Goldberg

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2 Scopus citations

Abstract

A procedure is described to quantitate inorganic phosphate in the form of the tris(trimethylsilyl) (TMS) phosphate by gas-liquid chromatography-mass spectrometry (glems) that increases the previously reported detection limit from the microgram to the nanogram range (i.e., 3 ng). The sensitivity for detecting TMS-phosphate by glc-ms analysis was shown to be limited by an increasing fractional loss with decreasing concentrations of TMS-phosphate analyzed due to its adsorption on different types of glc column supports. The method developed employs [18O4]phosphoric acid which serves as both an internal standard to permit quantitation and as a carrier to minimize sample adsorption on the glc column support.

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalAnalytical Biochemistry
Volume107
Issue number2
DOIs
StatePublished - 1980

Bibliographical note

Funding Information:
We would like to extend our gratitude to the University of Minnesota Agricultural Experiment Station and to Dr. R. T. Holman and Mr. W. Heimer-man (University of Minnesota, the Hormal Institute) for providing the glc-ms facilities. This research was supported by grants from U. S. Public Health Service, NS 05979; American Cancer Society, BC-166: and the Minnesota Leukemia Research Fund.

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