The use of Positional Scanning Synthetic Peptide Combinatorial Libraries To Identify Immunological Relevant Peptides

Mireia Sospedra, Clemencia Pinilla

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter discusses the underlying principles of the use of positional scanning synthetic combinatorial peptide libraries. The systematic identification of ligands for biologically relevant receptors has been greatly facilitated by the introduction of combinatorial peptide chemistry. Many receptor-ligand interactions in biology involve the binding of peptidic molecules to complex surface receptors. Among these, the immune system is a particularly interesting example, since peptides are the main targets for T lymphocytes and can also be recognized by antibodies. Synthetic combinatorial libraries (SCLs) made up of mixtures having one position defined with a given amino acid (aa), while other positions have amino acids incorporated as mixtures. These libraries represent a very large number of compounds such as synthetic peptides. SCLs are generated by use of the multiple solid phase synthesis method known as the "tea bag approach." The assay data derived from positional library provides information about the most important aa for every diversity position of the Positional Scanning (PS)-SCL.

Original languageEnglish (US)
Title of host publicationHandbook of Biologically Active Peptides
PublisherElsevier Inc.
Pages595-601
Number of pages7
ISBN (Print)9780123694423
DOIs
StatePublished - 2006
Externally publishedYes

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