Background: Anti-idiotypic antibodies (Ab-2), which are the mirror images of idiotypic antibodies (Ab-1), may be useful as diagnostic reagents and for use as immunogen to induce antigen-specific immune responses. Methods and Results: To explore the biologic potential of Ab-2 as diagnostic reagents in allergic diseases, murine mouse (m) Ab-2 were raised by immunizing Balb/c mice with affinity purified rabbit (r) Ab-1 specific for the pollen of Parthenium hysterophorus, an allergenic weed that grows wild on the Indian subcontinent and in Australia, Mexico, and the southern United States. Affinity purified Parthenium-specific human (h)AB-1 could successfully inhibit the binding of mAb-2 to immobilized rAb-1. Further, Balb/c mice immunized with mAb-2 induced Parthenium-specific anti-anti-idiotypic IgE and IgG antibodies. Specificity of the Ab-2 was confirmed by the ability of Parthenium pollen extracts to inhibit the binding of allergen-specific IgE and IgG Ab-1 in the sera of patients with rhinitis to immobilized mAb-2. Parthenium-sensitive patients with rhinitis who had positive results on skin prick tests to Parthenium pollen extracts also responded with a positive skin reaction to mAb-2. Conclusion: Our data demonstrate that Parthenium-specific mAb-2 may be of value as surrogate allergens in allergen standardization and for in vitro diagnosis.
Bibliographical noteFunding Information:
The airborne pollen of Parthenium hysteropho- rus (American feverfew), a member of the compositae f~mily, is a potent atopic allergen that is a common cause of allergic rhinitis in India. 1-3 Parthenium pollen allergens share cross-reactive From "the \]Laboratoryo f Immunology and Allergic Diseases, Departmqmt of Biochemistry, Indian Institute of Science, Bangalore, India, bthe Department of Medicine, St. Mar-tha's Ho.spital, Bangalore; and ~the Mast Cell Physiology Section, Laboratory of Clinical Investigation, National Insti-tute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md. Supported by grants from the Department of Biotechnology, Government of India, and the United States Agency for International Development under the Contraceptive Devel-opment •'r od Research in Immunology Program. Received fi)r publication Feb. 10, 1992; revised Mar. 2, 1993; accepted for publication Mar. 10, 1993. Reprint requests: Dr. P. Sriramarao, PhD, La Jolla Institute for Experimental Medicine, 11077 N. Torrey Pines Rd., La Jolla, CA 92037. Copyright ,~ 1993 by Mosby-Year Book, Inc. 0091-6749/93 $1.00 + .10 1/1/47124
- Anti-idiotypic antibodies
- skin tests