The uptake of native and desialylated glucocerebrosidase by rat hepatocytes and Kupffer cells

F. Scott Furbish, Clifford J. Steer, John A. Barranger, E. Anthony Jones, Roscoe O. Brady

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Human glucocerebrosidase is cleared from rat circulation after intravenous administration with a half-life of 21 minutes. The enzyme retains at least 85% of its activity following desialylation with neuraminidase and the half-life of the desialylated enzyme is 1.2 minutes. Asialo-orosomucoid inhibits the disappearance of the desialylated enzyme from the blood. Untreated enzyme is taken up by both Kupffer cells and hepatocytes while desialylated enzyme is cleared predominantly by hepatocytes and the uptake by Kupffer cells is reduced. These findings indicate that both Kupffer cells and hepatocytes take up a lysosomal enzyme and removal of sialic acid augments uptake by hepatocytes and decreases uptake by Kupffer cells.

Original languageEnglish (US)
Pages (from-to)1047-1053
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume81
Issue number3
DOIs
StatePublished - Apr 14 1978

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