The Ultrastructure of Human Organ-Cultured Cornea

I. Endothelium

Donald J Doughman, Diane Van Horn, John E. Harris, George E. Miller, Richard Lindstrom, Robert A. Good

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Human corneal endothelial cells maintained ultrastructural integrity after organ culture storage at 37 C for 10 to 21 days. Those organ-cultured corneas with postmortem times less than 12 hours had a complete layer of ultrastructurally intact endothelial cells of normal thickness and diameter. When postmortem times were longer than 24 hours, there was a complete layer of ultrastructurally intact endothelial cells that were large in diameter and thin. Control fellow corneas in moist chamber storage at 4 C demonstrated endothelial cell disruption proportional in area and severity to postmortem time duration. This implies that a dynamic process of endothelial repair, presumably by enlargement of remaining viable cells, occurs in organ culture.

Original languageEnglish (US)
Pages (from-to)516-523
Number of pages8
JournalArchives of Ophthalmology
Volume92
Issue number6
DOIs
StatePublished - Jan 1 1974

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Cornea
Endothelium
Endothelial Cells
Organ Culture Techniques

Cite this

Doughman, D. J., Van Horn, D., Harris, J. E., Miller, G. E., Lindstrom, R., & Good, R. A. (1974). The Ultrastructure of Human Organ-Cultured Cornea: I. Endothelium. Archives of Ophthalmology, 92(6), 516-523. https://doi.org/10.1001/archopht.1974.01010010530015

The Ultrastructure of Human Organ-Cultured Cornea : I. Endothelium. / Doughman, Donald J; Van Horn, Diane; Harris, John E.; Miller, George E.; Lindstrom, Richard; Good, Robert A.

In: Archives of Ophthalmology, Vol. 92, No. 6, 01.01.1974, p. 516-523.

Research output: Contribution to journalArticle

Doughman, DJ, Van Horn, D, Harris, JE, Miller, GE, Lindstrom, R & Good, RA 1974, 'The Ultrastructure of Human Organ-Cultured Cornea: I. Endothelium', Archives of Ophthalmology, vol. 92, no. 6, pp. 516-523. https://doi.org/10.1001/archopht.1974.01010010530015
Doughman DJ, Van Horn D, Harris JE, Miller GE, Lindstrom R, Good RA. The Ultrastructure of Human Organ-Cultured Cornea: I. Endothelium. Archives of Ophthalmology. 1974 Jan 1;92(6):516-523. https://doi.org/10.1001/archopht.1974.01010010530015
Doughman, Donald J ; Van Horn, Diane ; Harris, John E. ; Miller, George E. ; Lindstrom, Richard ; Good, Robert A. / The Ultrastructure of Human Organ-Cultured Cornea : I. Endothelium. In: Archives of Ophthalmology. 1974 ; Vol. 92, No. 6. pp. 516-523.
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