The Ultrastructure of Human Organ-Cultured Cornea: I. Endothelium

Donald J. Doughman, Diane Van Horn, John E. Harris, George E. Miller, Richard Lindstrom, Robert A. Good

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Human corneal endothelial cells maintained ultrastructural integrity after organ culture storage at 37 C for 10 to 21 days. Those organ-cultured corneas with postmortem times less than 12 hours had a complete layer of ultrastructurally intact endothelial cells of normal thickness and diameter. When postmortem times were longer than 24 hours, there was a complete layer of ultrastructurally intact endothelial cells that were large in diameter and thin. Control fellow corneas in moist chamber storage at 4 C demonstrated endothelial cell disruption proportional in area and severity to postmortem time duration. This implies that a dynamic process of endothelial repair, presumably by enlargement of remaining viable cells, occurs in organ culture.

Original languageEnglish (US)
Pages (from-to)516-523
Number of pages8
JournalArchives of Ophthalmology
Volume92
Issue number6
DOIs
StatePublished - Dec 1974

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