Abstract
Human corneal endothelial cells maintained ultrastructural integrity after organ culture storage at 37 C for 10 to 21 days. Those organ-cultured corneas with postmortem times less than 12 hours had a complete layer of ultrastructurally intact endothelial cells of normal thickness and diameter. When postmortem times were longer than 24 hours, there was a complete layer of ultrastructurally intact endothelial cells that were large in diameter and thin. Control fellow corneas in moist chamber storage at 4 C demonstrated endothelial cell disruption proportional in area and severity to postmortem time duration. This implies that a dynamic process of endothelial repair, presumably by enlargement of remaining viable cells, occurs in organ culture.
Original language | English (US) |
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Pages (from-to) | 516-523 |
Number of pages | 8 |
Journal | Archives of Ophthalmology |
Volume | 92 |
Issue number | 6 |
DOIs | |
State | Published - Dec 1974 |