The two-component system BfrAB regulates expression of ABC transporters in Streptococcus gordonii and Streptococcus sanguinis

Yongshu Zhang, Marvin Whiteley, Jens Kreth, Yu Lei, Ali Khammanivong, Jamie N. Evavold, Jingyuan Fan, Mark C Herzberg

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18 Citations (Scopus)

Abstract

The putative two-component system BfrAB is involved in Streptococcus gordonii biofilm development. Here, we provide evidence that BfrAB regulates the expression of bfrCD and bfrEFG, which encode two ATP-binding cassette (ABC) transporters, and bfrH, which encodes a CAAX amino-terminal protease family protein. BfrC and BfrE are ATP-binding proteins, and BfrD, BfrF and BfrG are homologous membrane-spanning polypeptides. Similarly, BfrABss, the BfrAB homologous system in Streptococcus sanguinis, controls the expression of two bfrCD-homologous operons (bfrCDss and bfrXYss), a bfrH-homologous gene (bfrH1ss) and another CAAX amino-terminal protease family protein gene (bfrH2ss). Furthermore, we demonstrate that the purified BfrA DNA-binding domain from S. gordonii binds to the promoter regions of bfrCD, bfrEFG, bfrH, bfrCDss, bfrXYss and bfrH1ss in vitro. Finally, we show that the BfrA DNA-binding domain recognizes a conserved DNA motif with a consensus sequence of TTTCTTTAGAAATATTTTAGAATT. These data suggest, therefore, that S. gordonii BfrAB controls biofilm formation by regulating multiple ABC-transporter systems.

Original languageEnglish (US)
Pages (from-to)165-173
Number of pages9
JournalMicrobiology
Volume155
Issue number1
DOIs
StatePublished - Apr 27 2009

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Streptococcus gordonii
ATP-Binding Cassette Transporters
Streptococcus
Biofilms
Peptide Hydrolases
Nucleotide Motifs
DNA
Consensus Sequence
Operon
Genetic Promoter Regions
Carrier Proteins
Proteins
Adenosine Triphosphate
Peptides
Membranes
Genes

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The two-component system BfrAB regulates expression of ABC transporters in Streptococcus gordonii and Streptococcus sanguinis. / Zhang, Yongshu; Whiteley, Marvin; Kreth, Jens; Lei, Yu; Khammanivong, Ali; Evavold, Jamie N.; Fan, Jingyuan; Herzberg, Mark C.

In: Microbiology, Vol. 155, No. 1, 27.04.2009, p. 165-173.

Research output: Contribution to journalArticle

Zhang, Yongshu ; Whiteley, Marvin ; Kreth, Jens ; Lei, Yu ; Khammanivong, Ali ; Evavold, Jamie N. ; Fan, Jingyuan ; Herzberg, Mark C. / The two-component system BfrAB regulates expression of ABC transporters in Streptococcus gordonii and Streptococcus sanguinis. In: Microbiology. 2009 ; Vol. 155, No. 1. pp. 165-173.
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