The transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputs

Leon Van Eck, Rebecca M. Davidson, Shuchi Wu, Bingyu Y. Zhao, Anna Maria Botha, Jan E. Leach, Nora L.V. Lapitan

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The transcription factor WRKY53 is expressed during biotic and abiotic stress responses in cereals, but little is currently known about its regulation, structure and downstream targets. We sequenced the wheat ortholog TaWRKY53 and its promoter region, which revealed extensive similarity in gene architecture and cis-acting regulatory elements to the rice ortholog OsWRKY53, including the presence of stress-responsive abscisic acid-responsive elements (ABRE) motifs and GCC-boxes. Four proteins interacted with the WRKY53 promoter in yeast one-hybrid assays, suggesting that this gene can receive inputs from diverse stress-related pathways such as calcium signalling and senescence, and environmental cues such as drought and ultraviolet radiation. The Ser/Thr receptor kinase ORK10/LRK10 and the apoplastic peroxidase POC1 are two downstream targets for regulation by the WRKY53 transcription factor, predicted based on the presence of W-box motifs in their promoters and coregulation with WRKY53, and verified by electrophoretic mobility shift assay (EMSA). Both ORK10/LRK10 and POC1 are upregulated during cereal responses to pathogens and aphids and important components of the oxidative burst during the hypersensitive response. Taken with our yeast two-hybrid assay which identified a strong protein-protein interaction between microsomal glutathione S-transferase 3 and WRKY53, this implies that the WRKY53 transcriptional network regulates oxidative responses to a wide array of stresses.

Original languageEnglish (US)
Pages (from-to)351-362
Number of pages12
JournalFunctional and Integrative Genomics
Volume14
Issue number2
DOIs
StatePublished - Jun 2014

Bibliographical note

Funding Information:
Acknowledgments We kindly thank Mariko Alexander for the assistance with the yeast-hybrid systems, Dr Marian Walhout (University of Massachusetts Medical School) for providing the yeast one-hybrid vectors and Dr Myron Bruce (USDA-ARS) for the helpful discussion. This study was supported by the US Department of Agriculture under the Cooperative Agreements USDA Contract No. 2009-34205-19960 and 2010-34205-21350 and Hatch Funds Project No. 644 to NL.

Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.

Keywords

  • Gene regulation
  • Oryza sativa
  • Plant disease resistance
  • Protein-DNA interactions
  • Triticum aestivum
  • WRKY transcription factors

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