The symbiovar trifolii of Rhizobium bangladeshense and Rhizobium aegyptiacum sp. nov. nodulate Trifolium alexandrinum in Egypt

Abdelaal Shamseldin; Lorena Carro; Alvaro Peix; Encarna Velázquez; Hassan Moawad; Michael J. Sadowsky

doi:10.1016/j.syapm.2016.05.002

The symbiovar trifolii of Rhizobium bangladeshense and Rhizobium aegyptiacum sp. nov. nodulate Trifolium alexandrinum in Egypt

Abdelaal Shamseldin, Lorena Carro, Alvaro Peix, Encarna Velázquez, Hassan Moawad, Michael J. Sadowsky

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Abstract

In the present work we analyzed the taxonomic status of several Rhizobium strains isolated from Trifolium alexandrinum L. nodules in Egypt. The 16S rRNA genes of these strains were identical to those of Rhizobium bangladeshense BLR175^T and Rhizobium binae BLR195^T. However, the analyses of recA and atpD genes split the strains into two clusters. Cluster II strains are identified as R. bangladeshense with >98% similarity values in both genes. The cluster I strains are phylogenetically related to Rhizobium etli CFN42^T and R. bangladeshense BLR175^T, but with less than 94% similarity values in recA and atpD genes. DNA-DNA hybridization analysis showed 42% and 48% average relatedness between the strain 1010^T from cluster I with respect to R. bangladeshense BLR175^T and R. etli CFN42^T, respectively. Phenotypic characteristics of cluster I strains also differed from those of their closest related Rhizobium species. Analysis of the nodC gene showed that the strains belong to two groups within the symbiovar trifolii which was identified in Egypt linked to the species R. bangladeshense. Based on the genotypic and phenotypic characteristics, the group I strains belong to a new species for which the name Rhizobium aegyptiacum sp. nov. (sv. trifolii) is proposed, with strain 1010^T being designated as the type strain (= USDA 7124^T = LMG 29296^T = CECT 9098^T).

Original language	English (US)
Pages (from-to)	275-279
Number of pages	5
Journal	Systematic and Applied Microbiology
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/j.syapm.2016.05.002
State	Published - Jun 1 2016

Bibliographical note

Funding Information:
This work was supported, in part, by funds from MINECO (Central Spanish Government) and by STDF project 1268 (Science Technology Development Fund) at the Academy of Scientific Research, Egypt , and the University of Minnesota Agricultural Experiment Station . We thank Dr. Aidan Parte for his help with the naming of this species.

Publisher Copyright:
© 2016 Elsevier GmbH.

Keywords

Egypt
Rhizobium
Rhizobium aegyptiacum
Rhizobium blangladeshense
Symbiovar trifolii
Trifolium alexandrinum

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10.1016/j.syapm.2016.05.002

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title = "The symbiovar trifolii of Rhizobium bangladeshense and Rhizobium aegyptiacum sp. nov. nodulate Trifolium alexandrinum in Egypt",

abstract = "In the present work we analyzed the taxonomic status of several Rhizobium strains isolated from Trifolium alexandrinum L. nodules in Egypt. The 16S rRNA genes of these strains were identical to those of Rhizobium bangladeshense BLR175T and Rhizobium binae BLR195T. However, the analyses of recA and atpD genes split the strains into two clusters. Cluster II strains are identified as R. bangladeshense with >98% similarity values in both genes. The cluster I strains are phylogenetically related to Rhizobium etli CFN42T and R. bangladeshense BLR175T, but with less than 94% similarity values in recA and atpD genes. DNA-DNA hybridization analysis showed 42% and 48% average relatedness between the strain 1010T from cluster I with respect to R. bangladeshense BLR175T and R. etli CFN42T, respectively. Phenotypic characteristics of cluster I strains also differed from those of their closest related Rhizobium species. Analysis of the nodC gene showed that the strains belong to two groups within the symbiovar trifolii which was identified in Egypt linked to the species R. bangladeshense. Based on the genotypic and phenotypic characteristics, the group I strains belong to a new species for which the name Rhizobium aegyptiacum sp. nov. (sv. trifolii) is proposed, with strain 1010T being designated as the type strain (= USDA 7124T = LMG 29296T = CECT 9098T).",

keywords = "Egypt, Rhizobium, Rhizobium aegyptiacum, Rhizobium blangladeshense, Symbiovar trifolii, Trifolium alexandrinum",

author = "Abdelaal Shamseldin and Lorena Carro and Alvaro Peix and Encarna Vel{\'a}zquez and Hassan Moawad and Sadowsky, {Michael J.}",

note = "Funding Information: This work was supported, in part, by funds from MINECO (Central Spanish Government) and by STDF project 1268 (Science Technology Development Fund) at the Academy of Scientific Research, Egypt , and the University of Minnesota Agricultural Experiment Station . We thank Dr. Aidan Parte for his help with the naming of this species. Publisher Copyright: {\textcopyright} 2016 Elsevier GmbH.",

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T1 - The symbiovar trifolii of Rhizobium bangladeshense and Rhizobium aegyptiacum sp. nov. nodulate Trifolium alexandrinum in Egypt

AU - Shamseldin, Abdelaal

AU - Carro, Lorena

AU - Peix, Alvaro

AU - Velázquez, Encarna

AU - Moawad, Hassan

AU - Sadowsky, Michael J.

N1 - Funding Information: This work was supported, in part, by funds from MINECO (Central Spanish Government) and by STDF project 1268 (Science Technology Development Fund) at the Academy of Scientific Research, Egypt , and the University of Minnesota Agricultural Experiment Station . We thank Dr. Aidan Parte for his help with the naming of this species. Publisher Copyright: © 2016 Elsevier GmbH.

PY - 2016/6/1

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N2 - In the present work we analyzed the taxonomic status of several Rhizobium strains isolated from Trifolium alexandrinum L. nodules in Egypt. The 16S rRNA genes of these strains were identical to those of Rhizobium bangladeshense BLR175T and Rhizobium binae BLR195T. However, the analyses of recA and atpD genes split the strains into two clusters. Cluster II strains are identified as R. bangladeshense with >98% similarity values in both genes. The cluster I strains are phylogenetically related to Rhizobium etli CFN42T and R. bangladeshense BLR175T, but with less than 94% similarity values in recA and atpD genes. DNA-DNA hybridization analysis showed 42% and 48% average relatedness between the strain 1010T from cluster I with respect to R. bangladeshense BLR175T and R. etli CFN42T, respectively. Phenotypic characteristics of cluster I strains also differed from those of their closest related Rhizobium species. Analysis of the nodC gene showed that the strains belong to two groups within the symbiovar trifolii which was identified in Egypt linked to the species R. bangladeshense. Based on the genotypic and phenotypic characteristics, the group I strains belong to a new species for which the name Rhizobium aegyptiacum sp. nov. (sv. trifolii) is proposed, with strain 1010T being designated as the type strain (= USDA 7124T = LMG 29296T = CECT 9098T).

AB - In the present work we analyzed the taxonomic status of several Rhizobium strains isolated from Trifolium alexandrinum L. nodules in Egypt. The 16S rRNA genes of these strains were identical to those of Rhizobium bangladeshense BLR175T and Rhizobium binae BLR195T. However, the analyses of recA and atpD genes split the strains into two clusters. Cluster II strains are identified as R. bangladeshense with >98% similarity values in both genes. The cluster I strains are phylogenetically related to Rhizobium etli CFN42T and R. bangladeshense BLR175T, but with less than 94% similarity values in recA and atpD genes. DNA-DNA hybridization analysis showed 42% and 48% average relatedness between the strain 1010T from cluster I with respect to R. bangladeshense BLR175T and R. etli CFN42T, respectively. Phenotypic characteristics of cluster I strains also differed from those of their closest related Rhizobium species. Analysis of the nodC gene showed that the strains belong to two groups within the symbiovar trifolii which was identified in Egypt linked to the species R. bangladeshense. Based on the genotypic and phenotypic characteristics, the group I strains belong to a new species for which the name Rhizobium aegyptiacum sp. nov. (sv. trifolii) is proposed, with strain 1010T being designated as the type strain (= USDA 7124T = LMG 29296T = CECT 9098T).

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The symbiovar trifolii of Rhizobium bangladeshense and Rhizobium aegyptiacum sp. nov. nodulate Trifolium alexandrinum in Egypt

Abstract

Bibliographical note

Keywords

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