Abstract
The specificity of 2,2,6,6-tetramethylpiperidine to singlet oxygen was shown using Rose Bengal as a singlet oxygen generator, and Xanthine-Xanthine Oxidase and KO2 as the sources for the superoxide radical. The highest concentration of produced-singlet oxygen occurred at 25% of O2 by Rose Bengal photosensitization. The linewidth of the EPR signal for photosensitized nitroxyl radical, increased with increasing oxygen content. The rate of nitroxyl radical formation increased with decreasing solvent polarity. Deuterated solvents enlarge the EPR signal intensity in a dose-dependant manner. No EPR signal increase was observed in xanthine-xanthine oxidase reaction or KO2 systems, indicating that TEMP does not react with the superoxide anion. Thus, the reaction of TEMP with 1O2 is highly specific.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 283-293 |
| Number of pages | 11 |
| Journal | Biochemistry and Molecular Biology International |
| Volume | 37 |
| Issue number | 2 |
| State | Published - 1995 |
Keywords
- EPR
- Rose Bengal
- Singlet oxygen
- Superoxide radical
- TEMP
- TEMPO