Abstract
This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.
Original language | English (US) |
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Article number | 85 |
Journal | Veterinary Sciences |
Volume | 8 |
Issue number | 5 |
DOIs | |
State | Published - May 2021 |
Bibliographical note
Funding Information:Funding: This research was funded by the Project of Antibiotic Stewardship in Companion Animal Practice jointly with Chiang Mai University/the University of Minnesota, College of Veterinary Medicine (R000012898), Excellent Center of Veterinary Public Health (R000016652), and Research Group for Veterinary Public Health (R000026062), Faculty of Veterinary Medicine, Chiang Mai University, Thailand.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
- MRS
- Pyoderma
- Skin swab
- Staphylococcal infection
- Type V SCCmec
- Zoonotic disease