The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas

Jaimee Reck, Alexandria M. Schauer, Kristyn Van Der Waal Mills, Raqual Bower, Douglas Tritschler, Catherine A. Perrone, Mary E. Porter

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18 Citations (Scopus)

Abstract

The assembly of cilia and flagella depends on the activity of two microtubule motor complexes, kinesin-2 and dynein-2/1b, but the specific functions of the different subunits are poorly defined. Here we analyze Chlamydomonas strains expressing different amounts of the dynein 1b light intermediate chain (D1bLIC). Disruption of D1bLIC alters the stability of the dynein 1b complex and reduces both the frequency and velocity of retrograde intraflagellar transport (IFT), but it does not eliminate retrograde IFT. Flagellar assembly, motility, gliding, and mating are altered in a dose-dependent manner. iTRAQ-based proteomics identifies a small subset of proteins that are significantly reduced or elevated in d1blic flagella. Transformation with D1bLIC-GFP rescues the mutant phenotypes, and D1bLIC-GFP assembles into the dynein 1b complex at wild-type levels. D1bLIC-GFP is transported with anterograde IFT particles to the flagellar tip, dissociates into smaller particles, and begins processive retrograde IFT in <2 s. These studies demonstrate the role of D1bLIC in facilitating the recycling of IFT subunits and other proteins, identify new components potentially involved in the regulation of IFT, flagellar assembly, and flagellar signaling, and provide insight into the role of D1bLIC and retrograde IFT in other organisms.

Original languageEnglish (US)
Pages (from-to)2404-2422
Number of pages19
JournalMolecular biology of the cell
Volume27
Issue number15
DOIs
StatePublished - Aug 1 2016

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Chlamydomonas
Dyneins
Flagella
Kinesin
Cilia
Protein Subunits
Recycling
Microtubules
Proteomics

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The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. / Reck, Jaimee; Schauer, Alexandria M.; Van Der Waal Mills, Kristyn; Bower, Raqual; Tritschler, Douglas; Perrone, Catherine A.; Porter, Mary E.

In: Molecular biology of the cell, Vol. 27, No. 15, 01.08.2016, p. 2404-2422.

Research output: Contribution to journalArticle

Reck, J, Schauer, AM, Van Der Waal Mills, K, Bower, R, Tritschler, D, Perrone, CA & Porter, ME 2016, 'The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas', Molecular biology of the cell, vol. 27, no. 15, pp. 2404-2422. https://doi.org/10.1091/mbc.E16-03-0191
Reck J, Schauer AM, Van Der Waal Mills K, Bower R, Tritschler D, Perrone CA et al. The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Molecular biology of the cell. 2016 Aug 1;27(15):2404-2422. https://doi.org/10.1091/mbc.E16-03-0191
Reck, Jaimee ; Schauer, Alexandria M. ; Van Der Waal Mills, Kristyn ; Bower, Raqual ; Tritschler, Douglas ; Perrone, Catherine A. ; Porter, Mary E. / The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. In: Molecular biology of the cell. 2016 ; Vol. 27, No. 15. pp. 2404-2422.
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