The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance

Alexander S. Zhovmer, Erdem D. Tabdanov, Houxun Miao, Han Wen, Jinqiu Chen, Xiaoling Luo, Xuefei Ma, Paolo P. Provenzano, Robert S. Adelstein

Research output: Contribution to journalArticle

Abstract

Contact guidance refers to the ability of cells to sense the geometrical features of the microenvironment and respond by changing their shape and adopting the appropriate orientation. Inhibition and ablation of nonmuscle myosin 2 (NM2) paralogues have demonstrated their importance for contact guidance. However, the specific roles of the NM2 paralogues have not been systematically studied. In this work we use micropatterned substrates to examine the roles of NM2A and NM2B and to elucidate the relationship of the microenvironment, actomyosin, and microtubules in contact guidance. We show that contact guidance is preserved following loss of NM2B and that expression of NM2A alone is sufficient to establish an appropriate orientation of the cells. Loss of NM2B and overexpression of NM2A result in a prominent cell polarization that is found to be linked to the increased alignment of microtubules with the actomyosin scaffold. Suppression of actomyosin with blebbistatin reduces cell polarity on a flat surface, but not on a surface with contact guidance cues. This indicates that the lost microtubule–actomyosin interactions are compensated for by microtubule–mi-croenvironment interactions, which are sufficient to establish cell polarity through contact guidance.

Original languageEnglish (US)
Pages (from-to)1961-1973
Number of pages13
JournalMolecular biology of the cell
Volume30
Issue number16
DOIs
StatePublished - Jul 22 2019

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Actomyosin
Myosins
Cell Polarity
Microtubules
Aptitude
Cues

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Zhovmer, A. S., Tabdanov, E. D., Miao, H., Wen, H., Chen, J., Luo, X., ... Adelstein, R. S. (2019). The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. Molecular biology of the cell, 30(16), 1961-1973. https://doi.org/10.1091/mbc.E19-01-0071

The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. / Zhovmer, Alexander S.; Tabdanov, Erdem D.; Miao, Houxun; Wen, Han; Chen, Jinqiu; Luo, Xiaoling; Ma, Xuefei; Provenzano, Paolo P.; Adelstein, Robert S.

In: Molecular biology of the cell, Vol. 30, No. 16, 22.07.2019, p. 1961-1973.

Research output: Contribution to journalArticle

Zhovmer, AS, Tabdanov, ED, Miao, H, Wen, H, Chen, J, Luo, X, Ma, X, Provenzano, PP & Adelstein, RS 2019, 'The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance', Molecular biology of the cell, vol. 30, no. 16, pp. 1961-1973. https://doi.org/10.1091/mbc.E19-01-0071
Zhovmer, Alexander S. ; Tabdanov, Erdem D. ; Miao, Houxun ; Wen, Han ; Chen, Jinqiu ; Luo, Xiaoling ; Ma, Xuefei ; Provenzano, Paolo P. ; Adelstein, Robert S. / The role of nonmuscle myosin 2A and 2B in the regulation of mesenchymal cell contact guidance. In: Molecular biology of the cell. 2019 ; Vol. 30, No. 16. pp. 1961-1973.
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