The role of mechanical forces in dextral rotation during cardiac looping in the chick embryo

Dmitry A. Voronov, Patrick W. Alford, Gang Xu, Larry A. Taber

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Cardiac looping is a vital morphogenetic process that transforms the initially straight heart tube into a curved tube normally directed toward the right side of the embryo. While recent work has brought major advances in our understanding of the genetic and molecular pathways involved in looping, the biophysical mechanisms that drive this process have remained poorly understood. This paper examines the role of biomechanical forces in cardiac rotation during the initial stages of looping, when the heart bends and rotates into a c-shaped tube (c-looping). Embryonic chick hearts were subjected to mechanical and chemical perturbations, and tissue stress and strain were studied using dissection and fluorescent labeling, respectively. The results suggest that (1) the heart contains little or no intrinsic ability to rotate, as external forces exerted by the splanchnopleure (SPL) and the omphalomesenteric veins (OVs) drive rotation; (2) unbalanced forces in the omphalomesenteric veins play a role in left-right looping directionality; and (3) in addition to ventral bending and rightward rotation, the heart tube also bends slightly toward the right. The results of this study may help investigators searching for the link between gene expression and the mechanical processes that drive looping.

Original languageEnglish (US)
Pages (from-to)339-350
Number of pages12
JournalDevelopmental Biology
Volume272
Issue number2
DOIs
StatePublished - Aug 15 2004

Bibliographical note

Funding Information:
This work was supported by NIH grant R01 HL64347 (LAT).

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Avian embryo
  • Biomechanics
  • Cardiac morphogenesis
  • Heart development
  • Left-right asymmetry

Fingerprint Dive into the research topics of 'The role of mechanical forces in dextral rotation during cardiac looping in the chick embryo'. Together they form a unique fingerprint.

Cite this