The role of Bcl-XS in radiation sensitivity

M. A. Morgan, J. N. Poynter, J. Maybaum, T. S. Lawrence

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Bcl-XS is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-XS overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-XS and reduced the surviving fraction of NIH 3T3 cells to 25%. However, radiation sensitivity was not significantly affected by Bcl-XS expression; the mean inactivation doses for Bcl-XS repressed and Bcl-XS induced cells were 2.7 ± 0.3 and 2.3 ± 0.1 Gy, respectively. We conclude that Bcl-XS induces cell death without affecting radiation sensitivity. These results suggest that mitochondrial pathways to apoptosis may not have a significant role in survival after irradiation.

Original languageEnglish (US)
Pages (from-to)535-539
Number of pages5
JournalRadiation research
Volume161
Issue number5
DOIs
StatePublished - May 2004

Fingerprint Dive into the research topics of 'The role of Bcl-X<sub>S</sub> in radiation sensitivity'. Together they form a unique fingerprint.

  • Cite this

    Morgan, M. A., Poynter, J. N., Maybaum, J., & Lawrence, T. S. (2004). The role of Bcl-XS in radiation sensitivity. Radiation research, 161(5), 535-539. https://doi.org/10.1667/RR3169