The role of Bcl-XS in radiation sensitivity

M. A. Morgan, J. N. Poynter, J. Maybaum, T. S. Lawrence

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Bcl-XS is a pro-apoptosis member of the Bcl2 family that has been shown to induce cell death and enhance chemosensitivity. We have investigated the effect of Bcl-XS overexpression on radiation sensitivity. Using a tetracycline-repressible system, we found that removal of tetracycline for 16 h induced Bcl-XS and reduced the surviving fraction of NIH 3T3 cells to 25%. However, radiation sensitivity was not significantly affected by Bcl-XS expression; the mean inactivation doses for Bcl-XS repressed and Bcl-XS induced cells were 2.7 ± 0.3 and 2.3 ± 0.1 Gy, respectively. We conclude that Bcl-XS induces cell death without affecting radiation sensitivity. These results suggest that mitochondrial pathways to apoptosis may not have a significant role in survival after irradiation.

Original languageEnglish (US)
Pages (from-to)535-539
Number of pages5
JournalRadiation research
Issue number5
StatePublished - May 2004


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