Ribosomes in lysates prepared from the mycelia of Aspergillus giganteus MDH 18894, which are actively secreting α-sarcin, do not contain the α-sarcin lesion. However, the addition of exogenous α-sarcin to these same lysates results in cleavage of the 26 S rRNA of the 60 S ribosomal subunit, characteristic of the cytotoxic action of α-sarcin. We conclude that A. giganteus ribosomes are not inherently resistant to the action of α-sarcin but are protected in vivo by producing α-sarcin in an inactive form and/or by the efficient cotranslational secretion of the toxin.
|Original language||English (US)|
|Number of pages||3|
|State||Published - Mar 14 1988|
Copyright 2014 Elsevier B.V., All rights reserved.
- (Aspergillus giganteus)
- Ribosome inactivation
- Ribosome-inactivating protein