The Rhodobacter capsulatus glnB gene is regulated by NtrC at tandem rpoN- independent promoters

D. Foster-Hartnett, R. G. Kranz

Research output: Contribution to journalComment/debate

30 Scopus citations

Abstract

The protein encoded by glnB of Rhodobacter capsulatus is part of a nitrogen-sensing cascade which regulates the expression of nitrogen fixation genes (nif). The expression of glnB was studied by using lacZ fusions, primer extension analysis, and in vitro DNase I footprinting. Our results suggest that glnB is transcribed from two promoters, one of which requires the R. capsulatus ntrC gene but is rpoN independent. Another promoter upstream of glnB is repressed by NtrC; purified R. capsulatus NtrC binds to sites that overlap this distal promoter region.

Original languageEnglish (US)
Pages (from-to)5171-5176
Number of pages6
JournalJournal of bacteriology
Volume176
Issue number16
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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