The reported cDNA sequence for phospholipase Cα encodes protein disulfide isomerase, isozyme Q-2 and not phospholipase-C

Sri P. Srivastava, Janies A. Fuchs, Jordan L. Holtzman

Research output: Contribution to journalArticlepeer-review

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Abstract

We have previously shown that the cDNA sequence published for the rat gene encoding phosphatidylinositol specific phospholipase Cα (1) may be identical to the sequence of a new isoform of protein disulfide isomerase, Isozyme Q2 (2). To determine whether the presumed phospholipase Cα gene encodes phospholipase C or protein disulfide isomerase, we have now expressed the cDNA in Escherichia coli and find that the 55.5 kDa protein that it synthesized contains no phopsholipase C activity. Moreover, an extract of Escherichia coli containing the expressed protein shows a concentration dependent increase in protein disulfide isomerase activity. The expressed protein is identical to protein disulfide isomerase, Isozyme Q2 on SDS-PAGE and by Western blot analysis. These current studies confirm that the published sequence of phospholipase Cα is, in fact, the sequence of protein disulfide isomerase, Isozyme Q2.

Original languageEnglish (US)
Pages (from-to)971-978
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume193
Issue number3
DOIs
StatePublished - Jun 30 1993

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