TY - JOUR
T1 - The relationship between proteases activity and glycoprotein levels in middle ear effusions from experimental otitis media in cats
AU - Hamaguchi, Yukiyoshi
AU - Takeuchi, Kazuhiko
AU - Jin, Chun Shun
AU - Majima, Yuichi
AU - Suzumura, Hidehisa
AU - Sakakura, Yasuo
AU - Juhn, Steven K.
N1 - Funding Information:
This work was supported by a Grant-in-aid for General Scientific Research (B) 62480355 from the Ministry of Education, Science and Culture of Japan.
PY - 1991
Y1 - 1991
N2 - The relationship between lysosomal proteases activity (elastase and cathepsin B) and levels of mucous glycoproteins in middle ear effusions (MEEs) was studied using a cat model of otitis media with effusion (OME) induced by Eustachian tube obstruction (ETO). The ratio of cathepsin B activity to total protein concentration (TPC) in MEE was 25.6 ±19.4 RFU/g×dl-1 at 1 week after ETO, and increased with the duration of OME. The ratio of elastase activity to TPC had a significant correlation to total leukocyte count. The ratio of fucose levels to TPC, which is one of the parameters reflecting levels of mucous glycoprotein, at 1 week after ETO was significantly higher than that at both 2 and 4 weeks after ETO. The percentage of glycoprotein levels absorbed to wheat germ lectin was highest at 1 week after ETO, and decreased with the duration of OME. In conclusion, mucous glycoproteins in cat occupy a larger portion of glycoproteins in MEE at the early stage of OME, and elastase and other lysosomal proteases may play a role in both stimulation of mucin release from goblet cells and mucin degradation. The balance of these processes seems to be a key factor determining mucin levels in MEEs.
AB - The relationship between lysosomal proteases activity (elastase and cathepsin B) and levels of mucous glycoproteins in middle ear effusions (MEEs) was studied using a cat model of otitis media with effusion (OME) induced by Eustachian tube obstruction (ETO). The ratio of cathepsin B activity to total protein concentration (TPC) in MEE was 25.6 ±19.4 RFU/g×dl-1 at 1 week after ETO, and increased with the duration of OME. The ratio of elastase activity to TPC had a significant correlation to total leukocyte count. The ratio of fucose levels to TPC, which is one of the parameters reflecting levels of mucous glycoprotein, at 1 week after ETO was significantly higher than that at both 2 and 4 weeks after ETO. The percentage of glycoprotein levels absorbed to wheat germ lectin was highest at 1 week after ETO, and decreased with the duration of OME. In conclusion, mucous glycoproteins in cat occupy a larger portion of glycoproteins in MEE at the early stage of OME, and elastase and other lysosomal proteases may play a role in both stimulation of mucin release from goblet cells and mucin degradation. The balance of these processes seems to be a key factor determining mucin levels in MEEs.
KW - Eustachian tube obstruction
KW - Experimental otitis media
KW - Lysosomal proteases
KW - Mucous glycoprotein
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U2 - 10.3109/00016489109127698
DO - 10.3109/00016489109127698
M3 - Article
C2 - 1862703
AN - SCOPUS:0025922298
SN - 0001-6489
VL - 111
SP - 23
EP - 29
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
IS - S483
ER -