The relationship between plasma free fatty acids and liver mitochondrial function in vivo

Ann M. Bode, Shere Byrd, Gary A. Klug

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


P O ratio, state 3 and 4 respiration rates, and acceptor control index (ACI) were assessed in rat liver mitochondria following an overnight fast and single bout of treadmill exercise of 30-180 min. P O was unaffected by fasting and 30 min of exercise; however, ACI was reduced because of an increase in state 4 respiration. Fasting, followed by running for 1 h or more decreased P O approx. 40% and ACI by 50%, an effect that could be attributed to a reduction in state 3 respiration. The decrease in P O was reversed 15 min after the cessation of exercise, whereas ACI remained depressed. All these functional alterations were mimicked by incubation of isolated mitochondria with palmitate and reversed by washing them with albumin. No direct correlation between plasma free fatty acids and the alterations in mitochondrial respiration was apparent. These data demonstrate that the decrease in the normal coupling of oxidation and phosphorylation in liver mitochondria produced by fasting /exercise is reversed rapidly in vivo. Furthermore, it is apparent that, if fatty acids act as a regulatory agent under these conditions, they do not do so solely on the basis of their plasma concentration.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Issue number2
StatePublished - Nov 12 1990
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by grants from the Medical Research Foundation of Oregon, the American Heart Association (Colorado Affiliate) and NIH (AR 39583).


  • Free fatty acid
  • Liver mitochondrial function
  • Mitochondrion
  • Plasma


Dive into the research topics of 'The relationship between plasma free fatty acids and liver mitochondrial function in vivo'. Together they form a unique fingerprint.

Cite this