The receptor-like cytoplasmic kinase PCRK1 contributes to pattern-triggered immunity against Pseudomonas syringae in Arabidopsis thaliana

Suma Sreekanta, Gerit Bethke, Noriyuki Hatsugai, Kenichi Tsuda, Amanda Thao, Lin Wang, Fumiaki Katagiri, Jane Glazebrook

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

In this paper we describe PATTERN-TRIGGERED IMMUNITY (PTI) COMPROMISED RECEPTOR-LIKE CYTOPLASMIC KINASE 1 (PCRK1) of Arabidopsis thaliana, an RLCK that is important for defense against the pathogen Pseudomonas syringae pv. maculicola ES4326 (Pma ES4326). We examined defense responses such as bacterial growth, production of reactive oxygen species (ROS) and callose deposition in pcrk1 mutant plants to determine the role of PCRK1 during pathogen infection. Expression of PCRK1 was induced following pathogen infection. Pathogen growth was significantly higher in pcrk1 mutant lines than in wild-type Col-0. Mutant pcrk1 plants showed reduced pattern-triggered immunity (PTI) against Pma ES4326 after pretreatment with peptides derived from flagellin (flg22), elongation factor-Tu (elf18), or an endogenous protein (pep1). Deposition of callose was reduced in pcrk1 plants, indicating a role of PCRK1 in activation of early immune responses. A PCRK1 transgene containing a mutation in a conserved lysine residue important for phosphorylation activity of kinases (K118E) failed to complement a pcrk1 mutant for the Pma ES4326 growth phenotype. Our study shows that PCRK1 plays an important role during PTI and that a conserved lysine residue in the putative kinase domain is important for PCRK1 function.

Original languageEnglish (US)
Pages (from-to)78-90
Number of pages13
JournalNew Phytologist
Volume207
Issue number1
DOIs
StatePublished - Jul 1 2015

Keywords

  • Arabidopsis
  • Flg22
  • Immunity
  • PTI COMPROMISED RECEPTOR-LIKE CYTOPLASMIC KINASE 1 (PCRK1)
  • Pathogen/microbe-associated molecular pattern (PAMP/MAMP)
  • Pattern-triggered immunity (PTI)
  • Pseudomonas syringae
  • Receptor-like cytoplasmic kinase

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