The Real Estate of Myoblast Cardiac Transplantation: Negative Remodeling Is Associated With Location

Jonathan D. McCue, Cory M Swingen, Tanya Feldberg, Gabe Caron, Adam Kolb, Christopher Denucci, Somnath Prabhu, Randy Motilall, Brian Breviu, Doris A. Taylor

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Background: Skeletal myoblast transplantation has been proposed as a therapy for ischemic cardiomyopathy owing to its possible role in myogenesis. The relative safety and efficacy based on location within scar is not known. We hypothesized that skeletal myoblasts transplanted into peripheral scar (compared with central scar) would more effectively attenuate negative left ventricular (LV) remodeling but at the risk of arrhythmia. Methods: New Zealand White rabbits (n = 34) underwent mid-left anterior descending artery (LAD) ligation to produce a transmural LV infarction. One month after LAD ligation, skeletal myoblasts were injected either in the scar center (n = 13) or scar periphery (n = 10) and compared with saline injection (n = 11). Holter monitoring and magnetic resonance imaging (MRI) was performed pre-injection; Holter monitoring was continued until 2 weeks after injection, with follow-up MRI at 1 month. Results: The centrally treated animals demonstrated increased LV end-systolic volume, end-diastolic volume, and mass that correlated with the number of injected cells. There was a trend toward attenuation of negative LV remodeling in peripherally treated animals compared with vehicle. Significant late ectopy was seen in several centrally injected animals, with no late ectopy seen in peripherally injected animals. Conclusions: We noted untoward effects with respect to negative LV remodeling after central injection, suggesting that transplanted cell location with respect to scar may be a key factor in the safety and efficacy of skeletal myoblast cardiac transplantation. Administration of skeletal myoblasts into peripheral scar appears safe, with a trend toward improved function in comparison with sham injection.

Original languageEnglish (US)
Pages (from-to)116-123
Number of pages8
JournalJournal of Heart and Lung Transplantation
Volume27
Issue number1
DOIs
StatePublished - Jan 2008

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    McCue, J. D., Swingen, C. M., Feldberg, T., Caron, G., Kolb, A., Denucci, C., Prabhu, S., Motilall, R., Breviu, B., & Taylor, D. A. (2008). The Real Estate of Myoblast Cardiac Transplantation: Negative Remodeling Is Associated With Location. Journal of Heart and Lung Transplantation, 27(1), 116-123. https://doi.org/10.1016/j.healun.2007.10.011