TY - JOUR
T1 - The rate of development of a microsporidan in moth cell culture
AU - Kurtti, T. J.
AU - Brooks, Marion A.
PY - 1977/3
Y1 - 1977/3
N2 - A microsporidan parasite of the forest tent caterpillar Malacosoma disstria infected cells and replicated in vitro in a line from the moth Heliothis zea. After spore germination, the incidence of infected cells increased with time until leveling off with sporulation. During the first 24 hr, there was a static number of parasites, followed by a 2-day logarithmic growth phase during which the population doubled five to six times. The growth rate was 9 to 11 hr per population doubling. Sporulation commenced on day 3, and 40 to 50 spores were recovered from each infected cell. The life cycle was completed within 6 days, culminating in spores that were infectious for cultured cells. The antibiotic fumagillin at a dose of 1 ppm in the culture medium was microsporida-static.
AB - A microsporidan parasite of the forest tent caterpillar Malacosoma disstria infected cells and replicated in vitro in a line from the moth Heliothis zea. After spore germination, the incidence of infected cells increased with time until leveling off with sporulation. During the first 24 hr, there was a static number of parasites, followed by a 2-day logarithmic growth phase during which the population doubled five to six times. The growth rate was 9 to 11 hr per population doubling. Sporulation commenced on day 3, and 40 to 50 spores were recovered from each infected cell. The life cycle was completed within 6 days, culminating in spores that were infectious for cultured cells. The antibiotic fumagillin at a dose of 1 ppm in the culture medium was microsporida-static.
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U2 - 10.1016/0022-2011(77)90184-7
DO - 10.1016/0022-2011(77)90184-7
M3 - Article
C2 - 403239
AN - SCOPUS:0017470443
VL - 29
SP - 126
EP - 132
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
SN - 0022-2011
IS - 2
ER -