The putative oncoprotein Bcl-3 induces cyclin D1 to stimulate G1 transition

S. D. Westerheide, M. W. Mayo, V. Anest, J. L. Hanson, Jr Baldwin

Research output: Contribution to journalArticlepeer-review

140 Scopus citations

Abstract

Bcl-3 is a distinctive member of the IκB family of NF-κB inhibitors because it can function to coactivate transcription. A potential involvement of Bcl-3 in oncogenesis is highlighted by the fact that it was cloned due to its location at a breakpoint junction in some cases of human B-cell chronic lymphocytic leukemia and that it is highly expressed in human breast tumor tissue. To analyze the effects of Bcl-3 dysregulation in breast epithelial cells, we created stable immortalized human breast epithelial cell lines either expressing Bcl-3 or carrying the corresponding vector control plasmid. Analysis of the Bcl-3-expressing cells suggests that these cells have a shortened G1 phase of the cell cycle as well as a significant increase in hyperphosphorylation of the retinoblastoma protein. Additionally, the cyclin D1 gene was found to be highly expressed in these cells. Upon further analysis, Bcl-3, acting as a coactivator with NF-κB p52 homodimers, was demonstrated to directly activate the cyclin D1 promoter through an NF-κB binding site. Therefore, our results demonstrate that dysregulated expression of Bcl-3 potentiates the G1 transition of the cell cycle by stimulating the transcription of the cyclin D1 gene in human breast epithelial cells.

Original languageEnglish (US)
Pages (from-to)8428-8436
Number of pages9
JournalMolecular and cellular biology
Volume21
Issue number24
DOIs
StatePublished - 2001

Fingerprint Dive into the research topics of 'The putative oncoprotein Bcl-3 induces cyclin D1 to stimulate G<sub>1</sub> transition'. Together they form a unique fingerprint.

Cite this