TY - JOUR
T1 - The pseudorabies virus VP1/2 tegument protein is required for intracellular capsid transport
AU - Luxton, G. W.Gant
AU - Lee, Joy I.Hsuan
AU - Haverlock-Moyns, Sarah
AU - Schober, Joseph Martin
AU - Smith, Gregory Allan
PY - 2006/1
Y1 - 2006/1
N2 - Transport of capsids in cells is critical to alphaherpesvirus infection and pathogenesis; however, viral factors required for transport have yet to be identified. Here we provide a detailed examination of capsid dynamics during the egress phase of infection in Vero cells infected with pseudorabies virus. We demonstrate that the VP1/2 tegument protein is required for processive microtubule-based transport of capsids in the cytoplasm. A second tegument protein that binds to VP1/2, UL37, was necessary for wild-type transport but was not essential for this process. Both proteins were also required for efficient nuclear egress of capsids to the cytoplasm.
AB - Transport of capsids in cells is critical to alphaherpesvirus infection and pathogenesis; however, viral factors required for transport have yet to be identified. Here we provide a detailed examination of capsid dynamics during the egress phase of infection in Vero cells infected with pseudorabies virus. We demonstrate that the VP1/2 tegument protein is required for processive microtubule-based transport of capsids in the cytoplasm. A second tegument protein that binds to VP1/2, UL37, was necessary for wild-type transport but was not essential for this process. Both proteins were also required for efficient nuclear egress of capsids to the cytoplasm.
UR - http://www.scopus.com/inward/record.url?scp=32944480977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=32944480977&partnerID=8YFLogxK
U2 - 10.1128/JVI.80.1.201-209.2006
DO - 10.1128/JVI.80.1.201-209.2006
M3 - Article
C2 - 16352544
AN - SCOPUS:32944480977
SN - 0022-538X
VL - 80
SP - 201
EP - 209
JO - Journal of virology
JF - Journal of virology
IS - 1
ER -