The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage

Jian Qu, Xian Ren, Rui ying Hou, Xing ping Dai, Ying chun Zhao, Xiao jing Xu, Wei Zhang, Gan Zhou, Hong hao Zhou, Zhao qian Liu

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Objectives: To investigate the effects of magnesium lithospermate B (LAB) on intracellular reactive oxygen species (ROS) production induced by high dose of glucose or H2O2, we explored the influences of LAB on the expression of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf2) in HEK293T cells after treatment with high dose of glucose. Materials and methods: The total nuclear proteins in HEK293T cells were extracted with Cytoplasmic Protein Extraction Kit. The ROS level was determined by flow cytometry. The mRNA and protein expression of HO-1 and Nrf2 were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results: LAB reduced the ROS production in HEK293T cells cultured under oxidative stress. High dose of glucose enhanced the expression of HO-1 mRNA and HO-1 protein in a time-dependent manner. LAB enhanced the expression of HO-1 mRNA and HO-1 protein in a dose-dependent manner treated with high dose of glucose. The amount of Nrf2 translocation was enhanced after cells were pretreated with 50μmol/L or 100μmol/L LAB. Silencing of Nrf2 gene eliminated the enhanced expression of HO-1 protein induced by high dose of glucose plus LAB. Conclusions: LAB plays an important role against glucose-induced intracellular oxidative damage. The enhanced expression of HO-1 mRNA and HO-1 protein caused by LAB is regulated via Nrf2 signal pathway.

Original languageEnglish (US)
Pages (from-to)32-39
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume411
Issue number1
DOIs
StatePublished - Jul 22 2011
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the National High-tech R&D Program of China (863 Program) ( 2009AA022703 , 2009AA022704 ), National Natural Science Foundation of China ( 30873089 ), Program for Changjiang Scholars and Innovative Research Team in University ( IRT0946 ), Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province, Open Foundation of Innovative Platform in University of Hunan Province of China ( 10K078 ), and the Science and Technology Plan Key Grant of Hunan Province of China ( 2009TP4068-2 ).

Keywords

  • HO-1
  • Magnesium lithospermate B
  • Nrf2
  • Reative oxygen species

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