The presence of an M4 subtype muscarinic receptor in the bovine adrenal medulla revealed by mRNA and receptor binding analyses

John C.R. Fernando, El Sayed A.M. Abdallah, Marian Evinger, Carlos Forray, Esam E. El-Fakahany

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The muscarinic receptor subtype present in the bovine adrenal medulla was characterized. Hybridization of RNA to highly specific m1-m5 muscarinic receptor cDNA probes detected the presence of only m4 subtype mRNA in this tissue. Muscarinic receptor binding studies using the non-selective ligand [3H]N-methyl-scopolamine showed a single class of binding sites with a maximum density of 19.8 fmol/mg protein and a dissociation constant (KD) of 220 pM in the adrenal medulla, while the M1 selective ligand [3H]telenzepine did not bind detectably. Competition of specific antagonists with [3H]N-methyl-scopolamine for binding to the membranes produced a rank order of potencies with a profile that fitted either the cloned m3 or m4 receptor. In further comparative studies, the adrenal gland of the rat showed the presence of m4 subtype mRNA in addition to the m3 subtype.

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume207
Issue number4
DOIs
StatePublished - Aug 14 1991

Keywords

  • (Bovine)
  • (Rat)
  • Adrenal medulla
  • Muscarinic receptors
  • mRNA

Fingerprint Dive into the research topics of 'The presence of an M<sub>4</sub> subtype muscarinic receptor in the bovine adrenal medulla revealed by mRNA and receptor binding analyses'. Together they form a unique fingerprint.

Cite this