The potential of Aedes triseriatus (Diptera: Culicidae) as an enzootic vector of West Nile virus

S. M. Erickson, K. B. Platt, B. J. Tucker, R. Evans, S. Tiawsirisup, W. A. Rowley

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The susceptibility of Aedes triseriatus (Say) (Diptera: Culicidae) to low levels of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) was determined and compared with that of Culex pipiens L. to assess the likelihood of its participation in an enzootic cycle involving mammals. Ae. triseriatus and Cx. pipiens were exposed to WNV by feeding on baby chickens with WNV serum titers ranging from 104.1 ± 0.1 to 10 8.6 ± 0.1 plaque-forming units (PFU)/ml and from 10 4.1 ± 0.1 to 107.0 PFU/ml, respectively. Infection rates and 95% confidence intervals (CIs) of 8% (4, 14) and 25% (15, 38) occurred in Ae. triseriatus and Cx. pipiens after feeding on chickens with WNV titers of 104.1 ± 0.1 PFU/ml and increased to 65% (49, 79) and 100% (72, 100) in Ae. triseriatus and Cx. pipiens after feeding on chickens with titers of 107.1 ± 0.1 PFU/ml. The mean infection rate of Ae. triseriatus ranged from 97% (84, 100) to 100% (79, 100) after feeding on chickens with WNV titers of ≥108.2 PFU/ml. The infectious dose (ID)50 values for Ae. triseriatus and Cx. pipiens were 10 6.5 (6.4, 6.7) and 104.9 (4.6, 5.1) PFU/ml, respectively. The combined estimated transmission rate of Ae. triseriatus at 14 and 18 d after feeding on chickens with a mean WNV titer of 108.6 ± 0.1 PFU/ml was 55%. Although Ae. triseriatus is significantly less susceptible to WNV than Cx. pipiens, the susceptibility of Ae. triseriatus to WNV titers <105.0 PFU/ml and its ability to transmit WNV suggest that Ae. triseriatus has the potential to be an enzootic vector among mammalian populations.

Original languageEnglish (US)
Pages (from-to)966-970
Number of pages5
JournalJournal of medical entomology
Volume43
Issue number5
DOIs
StatePublished - 2006
Externally publishedYes

Keywords

  • Aedes triseriatus
  • Culex pipiens
  • Vector competence
  • West Nile virus

Fingerprint

Dive into the research topics of 'The potential of Aedes triseriatus (Diptera: Culicidae) as an enzootic vector of West Nile virus'. Together they form a unique fingerprint.

Cite this