The physiological and mononuclear cell activation response to cryotherapy following a mixed martial arts contest: A pilot study

Angus Lindsay, Sam Carr, Mohd Izani Othman, Edward Marks, Sian Davies, Carl Petersen, Nick Draper, Steven P. Gieseg

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Cold water immersion is thought to reduce the inflammatory response to injury. Using cultured mononuclear cells and human subjects in a mixed martial arts (MMA) contest, we examined the effect of cryotherapy on 7,8-dihydroneopterin and neopterin generation. Urine was collected from 10 elite male mixed martial artists before, immediately post and 1, 2, 24 and 48 h following a contest. Myoglobin was analysed by reverse-phase high performance liquid chromatography, and urinary neopterin and total neopterin (neopterin+7,8-dihydroneopterin) were measured by strong cation exchange high-performance liquid chromatography. Cold water immersion and passive recovery were compared using changes in these markers, while cryotherapy tested total neopterin production in γ-interferon and phorbol myristate acetate (PMA)-stimulated blood-derived mononuclear cells (monocytes/T cells). Myoglobin significantly increased (p<0.05) at 1 h post-contest, neopterin significantly increased at 1 and 24 h (p<0.05), total neopterin significantly increased (p<0.05) at 1 h post for the passive group only, and significant individual variation was observed for all markers (p<0.01). Cold water immersion attenuated total neopterin production (p<0.05), while cryotherapy significantly reduced total neopterin production in PMA-stimulated mononuclear cells (p<0.01). Cryotherapy attenuates the post-exercise inflammatory response following an MMA contest. The evidence also suggests that the mechanisms responsible for this may be related to direct immune cell suppression.

Original languageEnglish (US)
Pages (from-to)143-151
Number of pages9
JournalPteridines
Volume26
Issue number4
DOIs
StatePublished - Dec 1 2015

Keywords

  • cold water immersion
  • inflammation
  • mixed martial arts
  • mononuclear cells
  • muscle damage

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