The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall

Shweta Chatrath; Vineet Kumar Gupta; Lalit C. Garg

doi:10.1016/j.febslet.2014.01.059

The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall

Shweta Chatrath
, Vineet Kumar Gupta
, Lalit C. Garg

Surgery

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30.

Original language	English (US)
Pages (from-to)	990-994
Number of pages	5
Journal	FEBS Letters
Volume	588
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2014.01.059
State	Published - Mar 18 2014

Bibliographical note

Funding Information:
Financial support from Department of Biotechnology, New Delhi , is acknowledged. The Council of Scientific and Industrial Research, New Delhi, is acknowledged for research fellowship to V.K.G. We thank Mr. K.P. Pandey for his technical assistance.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

C-terminal domain
Localization
Mycobacterium tuberculosis
PE domain
PE-PGRS30
PGRS domain

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10.1016/j.febslet.2014.01.059

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@article{a091da644ec64bd18778b170ec513c8f,

title = "The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall",

abstract = "PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30.",

keywords = "C-terminal domain, Localization, Mycobacterium tuberculosis, PE domain, PE-PGRS30, PGRS domain",

author = "Shweta Chatrath and Gupta, \{Vineet Kumar\} and Garg, \{Lalit C.\}",

note = "Funding Information: Financial support from Department of Biotechnology, New Delhi , is acknowledged. The Council of Scientific and Industrial Research, New Delhi, is acknowledged for research fellowship to V.K.G. We thank Mr. K.P. Pandey for his technical assistance.",

year = "2014",

month = mar,

day = "18",

doi = "10.1016/j.febslet.2014.01.059",

language = "English (US)",

volume = "588",

pages = "990--994",

journal = "FEBS Letters",

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T1 - The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall

AU - Chatrath, Shweta

AU - Gupta, Vineet Kumar

AU - Garg, Lalit C.

N1 - Funding Information: Financial support from Department of Biotechnology, New Delhi , is acknowledged. The Council of Scientific and Industrial Research, New Delhi, is acknowledged for research fellowship to V.K.G. We thank Mr. K.P. Pandey for his technical assistance.

PY - 2014/3/18

Y1 - 2014/3/18

N2 - PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30.

AB - PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30.

KW - C-terminal domain

KW - Localization

KW - Mycobacterium tuberculosis

KW - PE domain

KW - PE-PGRS30

KW - PGRS domain

UR - https://www.scopus.com/pages/publications/84896088203

UR - https://www.scopus.com/pages/publications/84896088203#tab=citedBy

U2 - 10.1016/j.febslet.2014.01.059

DO - 10.1016/j.febslet.2014.01.059

M3 - Article

C2 - 24530527

AN - SCOPUS:84896088203

SN - 0014-5793

VL - 588

SP - 990

EP - 994

JO - FEBS Letters

JF - FEBS Letters

IS - 6

ER -

The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall

Abstract

Bibliographical note

UN SDGs

Keywords

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