The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall

Shweta Chatrath, Vineet Kumar Gupta, Lalit C. Garg

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30.

Original languageEnglish (US)
Pages (from-to)990-994
Number of pages5
JournalFEBS Letters
Volume588
Issue number6
DOIs
StatePublished - Mar 18 2014

Bibliographical note

Funding Information:
Financial support from Department of Biotechnology, New Delhi , is acknowledged. The Council of Scientific and Industrial Research, New Delhi, is acknowledged for research fellowship to V.K.G. We thank Mr. K.P. Pandey for his technical assistance.

Keywords

  • C-terminal domain
  • Localization
  • Mycobacterium tuberculosis
  • PE domain
  • PE-PGRS30
  • PGRS domain

Fingerprint

Dive into the research topics of 'The PGRS domain is responsible for translocation of PE-PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall'. Together they form a unique fingerprint.

Cite this