PE-PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE-PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE-PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE-PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE-PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE-PGRS30.
Bibliographical noteFunding Information:
Financial support from Department of Biotechnology, New Delhi , is acknowledged. The Council of Scientific and Industrial Research, New Delhi, is acknowledged for research fellowship to V.K.G. We thank Mr. K.P. Pandey for his technical assistance.
- C-terminal domain
- Mycobacterium tuberculosis
- PE domain
- PGRS domain