The outer dynein arm-docking complex: Composition and characterization of a subunit (Oda1) necessary for outer arm assembly

Saeko Takada, Curtis G. Wilkerson, Ken Ichi Wakabayashi, Ritsu Kamiya, George B. Witman

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

To learn more about how dyneins are targeted to specific sites in the flagellum, we have investigated a factor necessary for binding of outer arm dynein to the axonemal microtubules of Chlamydomonas. This factor, termed the outer dynein arm-docking complex (ODA-DC), previously was shown to be missing from axonemes of the outer dynein armless mutants oda1 and oda3. We have now partially purified the ODA-DC, determined that it contains equimolar amounts of Mr ∼105,000 and ∼70,000 proteins plus a third protein of Mr ∼ 25,000, and found that it is associated with the isolated outer arm in a 1:1 molar ratio. We have cloned a full-length cDNA encoding the Mr ∼ 70,000 protein; the sequence predicts a 62.5-kDa protein with potential homologs in higher ciliated organisms, including humans. Sequencing of corresponding cDNA from strain oda1 revealed it has a mutation resulting in a stop codon just downstream of the initiator ATG; thus, it is unable to make the full-length Mr ∼ 70,000 protein. These results demonstrate that the ODA1 gene encodes the M r ∼ 70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule.

Original languageEnglish (US)
Pages (from-to)1015-1029
Number of pages15
JournalMolecular biology of the cell
Volume13
Issue number3
DOIs
StatePublished - 2002

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