The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter

Chatchai Chinpaisal, Liming Chang, Xinli Hu, Chih Hao Lee, Wu Nan Wen, Li-Na Wei

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The mouse orphan nuclear receptor TR2-11-f suppressed the expression of reporters fused to a hormone response element of the mouse cellular retinoic acid-binding protein I gene promoter. TR2-11 -f was able to bind to a direct repeat with four nucleotides in the spacer (5'TGACCTTTGGGGACCT3') located within this hormone response element as homodimers. The specificity of protein-DNA interactions was demonstrated by competition in gel retardation and antibody-mediated supershift reactions. The residues critical for TR2- 11-f binding were mapped to both repeated sequences, whereas the spacer and the flanking sequences were less important. The K(d) and B(max) of TR2-11-f homodimer binding to this direct repeat were determined to be 2.6 nM and 0.012 nM, respectively. By using a yeast two-hybrid system, it was demonstrated that dimerization of TR2-11-f was mediated by its ligand- binding domain. The actions of TR2-11-f in regulating cellular retinoic acid- binding protein 1 gene will likely influence retinoic action and availability within the cells.

Original languageEnglish (US)
Pages (from-to)14088-14095
Number of pages8
JournalBiochemistry
Volume36
Issue number46
DOIs
StatePublished - Nov 18 1997

Fingerprint Dive into the research topics of 'The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter'. Together they form a unique fingerprint.

Cite this