The oncogenic response to MiR-335 is associated with cell surface expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) activity

Fausto Rojas, Maria E. Hernandez, Milagros Silva, LIHUA LI, Subree Subramanian, Michael J Wilson, Ping Liu

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Abstract

MicroRNA miR-335 has been reported to have both tumor suppressor and oncogenic activities. In order to determine possible tissue and cell type differences in response to miR-335, we examined the effect of miR-335 on cell expression of MT1-MMP, a proteinase commonly expressed in tumors and associated with cell proliferation and migration. miR-335 increased cell surface expression of MT1-MMP in fibrosarcoma HT-1080 and benign prostate BPH-1 cells, but not in prostate LNCaP or breast MCF-7 tumor cells. miR-335 stimulated proliferation and cell migration in a wound healing in vitro assay in HT-1080, BPH-1, and U87 glioblastoma cells, cells which demonstrated significant cell surface expression of MT1-MMP. In contrast, miR-335 did not affect proliferation or migration in cells without a prominent plasma membrane associated MT1-MMP activity. Our data suggest that differences in response to miR-335 by tumor cells may lie in part in the mechanism of regulation of MT1-MMP production.

Original languageEnglish (US)
Article numbere0132026
JournalPloS one
Volume10
Issue number7
DOIs
StatePublished - Jul 23 2015

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interstitial collagenase
Matrix Metalloproteinase 14
Tumors
cell movement
Cell Movement
cells
Cells
Prostate
Neoplasms
cell proliferation
Membrane-Associated Matrix Metalloproteinases
Cell proliferation
Cell membranes
Cell Membrane
MicroRNAs
fibrosarcoma
neoplasms
Assays
Fibrosarcoma
Peptide Hydrolases

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The oncogenic response to MiR-335 is associated with cell surface expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) activity. / Rojas, Fausto; Hernandez, Maria E.; Silva, Milagros; LI, LIHUA; Subramanian, Subree; Wilson, Michael J; Liu, Ping.

In: PloS one, Vol. 10, No. 7, e0132026, 23.07.2015.

Research output: Contribution to journalArticle

Rojas, Fausto ; Hernandez, Maria E. ; Silva, Milagros ; LI, LIHUA ; Subramanian, Subree ; Wilson, Michael J ; Liu, Ping. / The oncogenic response to MiR-335 is associated with cell surface expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) activity. In: PloS one. 2015 ; Vol. 10, No. 7.
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