The oncogenic response to MiR-335 is associated with cell surface expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) activity

Fausto Rojas, Maria E. Hernandez, Milagros Silva, Lihua Li, Subbaya Subramanian, Michael J. Wilson, Ping Liu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

MicroRNA miR-335 has been reported to have both tumor suppressor and oncogenic activities. In order to determine possible tissue and cell type differences in response to miR-335, we examined the effect of miR-335 on cell expression of MT1-MMP, a proteinase commonly expressed in tumors and associated with cell proliferation and migration. miR-335 increased cell surface expression of MT1-MMP in fibrosarcoma HT-1080 and benign prostate BPH-1 cells, but not in prostate LNCaP or breast MCF-7 tumor cells. miR-335 stimulated proliferation and cell migration in a wound healing in vitro assay in HT-1080, BPH-1, and U87 glioblastoma cells, cells which demonstrated significant cell surface expression of MT1-MMP. In contrast, miR-335 did not affect proliferation or migration in cells without a prominent plasma membrane associated MT1-MMP activity. Our data suggest that differences in response to miR-335 by tumor cells may lie in part in the mechanism of regulation of MT1-MMP production.

Original languageEnglish (US)
Article numbere0132026
JournalPloS one
Volume10
Issue number7
DOIs
StatePublished - Jul 23 2015

Bibliographical note

Publisher Copyright:
© 2015 Rojas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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