The Oligosaccharyltransferase AglB Supports SurfaceAssociated Growth and Iron Oxidation in Methanococcus maripaludis

Matthew P. Holten, Dallas R. Fonseca, Kyle C. Costaa

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Most microbial organisms grow as surface-attached communities known as biofilms. However, the mechanisms whereby methanogenic archaea grow attached to surfaces have remained understudied. Here, we show that the oligosaccharyltransferase AglB is essential for growth of Methanococcus maripaludis strain JJ on glass or metal surfaces. AglB glycosylates several cellular structures, such as pili, archaella, and the cell surface layer (S-layer). We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion of aglB blocked S-layer glycosylation and abolished surface-associated growth. A strain JJ mutant lacking structural components of the type IV-like pilus did not have a growth defect under any conditions tested, while a mutant lacking the preflagellin peptidase (Δ flaK) was defective for surface growth only when formate was provided as the sole electron donor. Finally, for strains that are capable of Fe 0 oxidation, we show that deletion of aglB decreases the rate of anaerobic Fe 0 oxidation, presumably due to decreased association of biomass with the Fe 0 surface. Together, these data provide an initial characterization of surface-associated growth in a member of the methanogenic archaea. IMPORTANCE Methanogenic archaea are responsible for producing the majority of methane on Earth and catalyze the terminal reactions in the degradation of organic matter in anoxic environments. Methanogens often grow as biofilms associated with surfaces or partner organisms; however, the molecular details of surface-associated growth remain uncharacterized. We have found evidence that glycosylation of the cell surface layer is essential for growth of M. maripaludis on surfaces and can enhance rates of anaerobic iron corrosion. These results provide insight into the physiology of surface-associated methanogenic organisms and highlight the importance of surface association for anaerobic iron corrosion.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalApplied and environmental microbiology
Volume87
Issue number17
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
This work was sponsored by a Young Investigator Program award to K.C.C. from the Army Research Office, grant number W911NF-19-1-0024. D.R.F. was supported by the National Science Foundation Graduate Research Fellowship Program under grant number CON-75851.

Publisher Copyright:
Copyright © 2021 American Society for Microbiology. All Rights Reserved.

Keywords

  • Archaea
  • Methanococcus
  • N-linked glycosylation
  • biofilm
  • iron oxidation
  • Archaeal Proteins/genetics
  • Oxidation-Reduction
  • Hexosyltransferases/genetics
  • Membrane Proteins/genetics
  • Glycosylation
  • Methane/metabolism
  • Methanococcus/enzymology
  • Iron/metabolism

PubMed: MeSH publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Journal Article

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