The number of Oesophagostomum spp. larvae recovered in coprocultures varies with media used

Alexander D. Hernandez, Billie Kantner, Emely Santos, Sara Major, Rick Carr, Yuzhi Li

Research output: Contribution to journalArticlepeer-review


Nodular roundworms (Oesophagostomum spp.) are frequent parasites of the large intestine in several mammal species including humans and pigs, and their study often requires the use of infective larvae produced using several coproculture techniques. However, there is no published comparison of techniques to determine which yields the highest number of larvae. This study compares the number of larvae recovered from coprocultures made with charcoal, sawdust, vermiculite, and water in an experiment repeated twice using feces from a sow naturally infected with Oesophagostomum spp. at an organic farm. A higher number of larvae were recovered from coprocultures using sawdust relative to other types of media used, and this was consistent across the two trials. The use of sawdust to culture Oesophagostomum spp. larvae is rarely reported and our study suggests it can yield higher numbers relative to other media.

Original languageEnglish (US)
Article numbere55
JournalJournal of Helminthology
StatePublished - Jul 10 2023

Bibliographical note

Publisher Copyright:
© The Author(s), 2023. Published by Cambridge University Press.


  • coproculture
  • intestinal parasites
  • nematode
  • nodular worms
  • organic pig
  • sawdust

PubMed: MeSH publication types

  • Journal Article


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