The nucleotide sequence of a mitochondrial replicon from maize

Steven R. Ludwig; Robert F. Pohlman; Jeffrey Vieira; Alan G. Smith; Joachim Messing

doi:10.1016/0378-1119(85)90211-2

The nucleotide sequence of a mitochondrial replicon from maize

Steven R. Ludwig
, Robert F. Pohlman
, Jeffrey Vieira
, Alan G. Smith
, Joachim Messing

Horticultural Science

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The 1913-bp maize mitochondrial (mt) plasmid was isolated from a suspension culture of a Black Mexican Sweet maize strain, cloned into M13mp vectors, and sequenced by a unidirectional progressive deletion method. The 1.9-kb extrachromosomal double-stranded circular DNA plasmid was found to contain regions of sequence which in other systems are known to be part of origins of replication (ori). This plasmid could be used as a carrier for chimeric genes and a molecular probe for replication.

Original language	English (US)
Pages (from-to)	131-138
Number of pages	8
Journal	Gene
Volume	38
Issue number	1-3
DOIs	https://doi.org/10.1016/0378-1119(85)90211-2
State	Published - 1985

Bibliographical note

Funding Information:
We wouldl ike to thankK ris Kohn and Stephanie Young for their help in preparingt he manuscript, also MichaelZ arowitz,P eterW einsteina, ndA rthur Weissingerfo r their helpfuld iscussionsT. his work was supportedb y NIH grantN o. GM3 1499to J.M., while R.F.P. is a recipiento f a NSF postdoctoral fellowship in plant molecular biology (PCM-831 2545)a, ndJ .V. is supportedb y theN IH training grantN o. ST32GM107094.

Keywords

Cloning vector
gene transfer
molecular probe
plasmid
recombinant DNA

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10.1016/0378-1119(85)90211-2

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title = "The nucleotide sequence of a mitochondrial replicon from maize",

abstract = "The 1913-bp maize mitochondrial (mt) plasmid was isolated from a suspension culture of a Black Mexican Sweet maize strain, cloned into M13mp vectors, and sequenced by a unidirectional progressive deletion method. The 1.9-kb extrachromosomal double-stranded circular DNA plasmid was found to contain regions of sequence which in other systems are known to be part of origins of replication (ori). This plasmid could be used as a carrier for chimeric genes and a molecular probe for replication.",

keywords = "Cloning vector, gene transfer, molecular probe, plasmid, recombinant DNA",

author = "Ludwig, \{Steven R.\} and Pohlman, \{Robert F.\} and Jeffrey Vieira and Smith, \{Alan G.\} and Joachim Messing",

note = "Funding Information: We wouldl ike to thankK ris Kohn and Stephanie Young for their help in preparingt he manuscript, also MichaelZ arowitz,P eterW einsteina, ndA rthur Weissingerfo r their helpfuld iscussionsT. his work was supportedb y NIH grantN o. GM3 1499to J.M., while R.F.P. is a recipiento f a NSF postdoctoral fellowship in plant molecular biology (PCM-831 2545)a, ndJ .V. is supportedb y theN IH training grantN o. ST32GM107094.",

year = "1985",

doi = "10.1016/0378-1119(85)90211-2",

language = "English (US)",

volume = "38",

pages = "131--138",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

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T1 - The nucleotide sequence of a mitochondrial replicon from maize

AU - Ludwig, Steven R.

AU - Pohlman, Robert F.

AU - Vieira, Jeffrey

AU - Smith, Alan G.

AU - Messing, Joachim

N1 - Funding Information: We wouldl ike to thankK ris Kohn and Stephanie Young for their help in preparingt he manuscript, also MichaelZ arowitz,P eterW einsteina, ndA rthur Weissingerfo r their helpfuld iscussionsT. his work was supportedb y NIH grantN o. GM3 1499to J.M., while R.F.P. is a recipiento f a NSF postdoctoral fellowship in plant molecular biology (PCM-831 2545)a, ndJ .V. is supportedb y theN IH training grantN o. ST32GM107094.

PY - 1985

Y1 - 1985

N2 - The 1913-bp maize mitochondrial (mt) plasmid was isolated from a suspension culture of a Black Mexican Sweet maize strain, cloned into M13mp vectors, and sequenced by a unidirectional progressive deletion method. The 1.9-kb extrachromosomal double-stranded circular DNA plasmid was found to contain regions of sequence which in other systems are known to be part of origins of replication (ori). This plasmid could be used as a carrier for chimeric genes and a molecular probe for replication.

AB - The 1913-bp maize mitochondrial (mt) plasmid was isolated from a suspension culture of a Black Mexican Sweet maize strain, cloned into M13mp vectors, and sequenced by a unidirectional progressive deletion method. The 1.9-kb extrachromosomal double-stranded circular DNA plasmid was found to contain regions of sequence which in other systems are known to be part of origins of replication (ori). This plasmid could be used as a carrier for chimeric genes and a molecular probe for replication.

KW - Cloning vector

KW - gene transfer

KW - molecular probe

KW - plasmid

KW - recombinant DNA

UR - https://www.scopus.com/pages/publications/0022310728

UR - https://www.scopus.com/pages/publications/0022310728#tab=citedBy

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DO - 10.1016/0378-1119(85)90211-2

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AN - SCOPUS:0022310728

SN - 0378-1119

VL - 38

SP - 131

EP - 138

JO - Gene

JF - Gene

IS - 1-3

ER -

The nucleotide sequence of a mitochondrial replicon from maize

Abstract

Bibliographical note

Keywords

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