Abstract
The cell-free molecular synthesis of biochemical systems is a rapidly growing field of research. Advances in the Human Genome Project, DNA synthesis, and other technologies have allowed the in vitro construction of biochemical systems, termed cell-free biology, to emerge as an exciting domain of bioengineering. Cell-free biology ranges from the molecular to the cell-population scales, using an ever-expanding variety of experimental platforms and toolboxes. In this review, we discuss the ongoing efforts undertaken in the three major classes of cell-free biology methodologies, namely protein-based, nucleic acids-based, and cell-free transcription-translation systems, and provide our perspectives on the current challenges as well as the major goals in each of the subfields.
Original language | English (US) |
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Pages (from-to) | 51-77 |
Number of pages | 27 |
Journal | Annual Review of Biomedical Engineering |
Volume | 22 |
DOIs | |
State | Published - Jun 4 2020 |
Bibliographical note
Publisher Copyright:© 2020 by Annual Reviews. All rights reserved.
Keywords
- Bottom-up synthetic biology
- cell-free transcription-translation
- self-assembly
- synthetic cell