The cell-free molecular synthesis of biochemical systems is a rapidly growing field of research. Advances in the Human Genome Project, DNA synthesis, and other technologies have allowed the in vitro construction of biochemical systems, termed cell-free biology, to emerge as an exciting domain of bioengineering. Cell-free biology ranges from the molecular to the cell-population scales, using an ever-expanding variety of experimental platforms and toolboxes. In this review, we discuss the ongoing efforts undertaken in the three major classes of cell-free biology methodologies, namely protein-based, nucleic acids-based, and cell-free transcription-translation systems, and provide our perspectives on the current challenges as well as the major goals in each of the subfields.
|Original language||English (US)|
|Number of pages||27|
|Journal||Annual Review of Biomedical Engineering|
|State||Published - Jun 4 2020|
Bibliographical notePublisher Copyright:
© 2020 by Annual Reviews. All rights reserved.
- Bottom-up synthetic biology
- cell-free transcription-translation
- synthetic cell
PubMed: MeSH publication types
- Journal Article
- Research Support, U.S. Gov't, Non-P.H.S.
- Research Support, Non-U.S. Gov't