TY - JOUR
T1 - The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro
AU - Punzel, M.
AU - Wissink, S. D.
AU - Miller, J. S.
AU - Moore, K. A.
AU - Lemischka, I. R.
AU - Verfaillie, C. M.
PY - 1999/6/1
Y1 - 1999/6/1
N2 - Hematopoietic stem cells (HSC) are cells with self-renewing multilineage differentiation potential. Although engraftment in xenogeneic recipients can be used to measure human HSC, these assays do not allow assessment of individual progenitors. We developed an in vitro assay that allows the identification of a single human bone marrow progenitor closely related to HSC, which we termed 'Myeloid-Lymphoid Initiating Cell,' or ML-IC, because it is capable of generating multiple secondary progenitors that can reinitiate long-term myeloid and lymphoid hematopoiesis in vitro. The assay is done in contact with murine AFT024 fetal liver stromal cells and with Flt3-Ligand, stem cell factor, and interleukin-7. In this assay, 0.2% to 1.7% of Lin - /34+/DR(dim) cells could generate 1 to 3 long-term culture initiating cells (LTC-IC) as well as I to 4 NK-IC after 4 to 6 weeks. In addition, this assay measures contribution of net-progenitor conservation and net-progenitor proliferation over time, providing insight in the fate of individual LTC-IC and NK-IC. This assay will prove useful to enumerate the number of very primitive human progenitors with multilineage differentiation potential, as well as to evaluate future ex vivo culture conditions.
AB - Hematopoietic stem cells (HSC) are cells with self-renewing multilineage differentiation potential. Although engraftment in xenogeneic recipients can be used to measure human HSC, these assays do not allow assessment of individual progenitors. We developed an in vitro assay that allows the identification of a single human bone marrow progenitor closely related to HSC, which we termed 'Myeloid-Lymphoid Initiating Cell,' or ML-IC, because it is capable of generating multiple secondary progenitors that can reinitiate long-term myeloid and lymphoid hematopoiesis in vitro. The assay is done in contact with murine AFT024 fetal liver stromal cells and with Flt3-Ligand, stem cell factor, and interleukin-7. In this assay, 0.2% to 1.7% of Lin - /34+/DR(dim) cells could generate 1 to 3 long-term culture initiating cells (LTC-IC) as well as I to 4 NK-IC after 4 to 6 weeks. In addition, this assay measures contribution of net-progenitor conservation and net-progenitor proliferation over time, providing insight in the fate of individual LTC-IC and NK-IC. This assay will prove useful to enumerate the number of very primitive human progenitors with multilineage differentiation potential, as well as to evaluate future ex vivo culture conditions.
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U2 - 10.1182/blood.v93.11.3750.411a37_3750_3756
DO - 10.1182/blood.v93.11.3750.411a37_3750_3756
M3 - Article
C2 - 10339481
AN - SCOPUS:0033153096
SN - 0006-4971
VL - 93
SP - 3750
EP - 3756
JO - Blood
JF - Blood
IS - 11
ER -