The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2

Milena Edna Roux, Magnus Wohlfahrt Rasmussen, Kristoffer Palma, Signe Lolle, Àngels Mateu Regué, Gerit Bethke, Jane Glazebrook, Weiping Zhang, Leslie Sieburth, Martin R. Larsen, John Mundy, Morten Petersen

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Multi-layered defense responses are activated in plants upon recognition of invading pathogens. Transmembrane receptors recognize conserved pathogen-associated molecular patterns (PAMPs) and activate MAP kinase cascades, which regulate changes in gene expression to produce appropriate immune responses. For example, Arabidopsis MAP kinase 4 (MPK4) regulates the expression of a subset of defense genes via at least one WRKY transcription factor. We report here that MPK4 is found in complexes in vivo with PAT1, a component of the mRNA decapping machinery. PAT1 is also phosphorylated by MPK4 and, upon flagellin PAMP treatment, PAT1 accumulates and localizes to cytoplasmic processing (P) bodies which are sites for mRNA decay. Pat1 mutants exhibit dwarfism and de-repressed immunity dependent on the immune receptor SUMM2. Since mRNA decapping is a critical step in mRNA turnover, linking MPK4 to mRNA decay via PAT1 provides another mechanism by which MPK4 may rapidly instigate immune responses. Synopsis The identification of mRNA decapping factor PAT1 as a new target for MAP kinase 4 offers insight on the emerging connection between RNA metabolism and immunity in plants. Eukaryotic PAT1 proteins are key components in post-transcriptional regulation of gene expression Arabidopsis PAT1 functions in decapping and is a substrate of MAP kinase 4 which regulates immune responses in a PAMP-triggered pathway associated with the immune receptor SUMM2 PAT1 accumulates in processing (P) bodies upon PAMP treatment PAT1 interacts with the resistance protein SUMM2 and pat1 mutants exhibit SUMM2-dependent autoimmunity in Arabidopsis The identification of mRNA decapping factor PAT1 as a new target for MAP kinase 4 offers insight on the emerging connection between RNA metabolism and immunity in plants.

Original languageEnglish (US)
Pages (from-to)593-608
Number of pages16
JournalEMBO Journal
Volume34
Issue number5
DOIs
StatePublished - Mar 4 2015

Keywords

  • MAP kinases
  • decapping
  • immunity
  • mRNA decay
  • phosphorylation

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    Roux, M. E., Rasmussen, M. W., Palma, K., Lolle, S., Regué, À. M., Bethke, G., Glazebrook, J., Zhang, W., Sieburth, L., Larsen, M. R., Mundy, J., & Petersen, M. (2015). The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2. EMBO Journal, 34(5), 593-608. https://doi.org/10.15252/embj.201488645