The molecular landscape of the University of Michigan laryngeal squamous cell carcinoma cell line panel

Jacqueline E. Mann; Aditi Kulkarni; Andrew C. Birkeland; Judy Kafelghazal; Julia Eisenberg; Brittany M. Jewell; Megan L. Ludwig; Matthew E. Spector; Hui Jiang; Thomas E. Carey; J. Chad Brenner

doi:10.1002/hed.25803

The molecular landscape of the University of Michigan laryngeal squamous cell carcinoma cell line panel

Jacqueline E. Mann
, Aditi Kulkarni
, Andrew C. Birkeland
, Judy Kafelghazal
, Julia Eisenberg
, Brittany M. Jewell
, Megan L. Ludwig
, Matthew E. Spector
, Hui Jiang
, Thomas E. Carey
, J. Chad Brenner

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Background: Laryngeal squamous cell carcinomas (LSCCs) have a high risk of recurrence and poor prognosis. Patient-derived cancer cell lines remain important preclinical models for advancement of new therapeutic strategies, and comprehensive characterization of these models is vital in the precision medicine era. Methods: We performed exome and transcriptome sequencing as well as copy number analysis of a panel of LSCC-derived cell lines that were established at the University of Michigan and are used in laboratories worldwide. Results: We observed a complex array of alterations consistent with those reported in The Cancer Genome Atlas head and neck squamous cell carcinoma project, including aberrations in PIK3CA, EGFR, CDKN2A, TP53, and NOTCH family and FAT1 genes. A detailed analysis of FAT family genes and associated pathways showed disruptions to these genes in most cell lines. Conclusions: The molecular profiles we have generated indicate that as a whole, this panel recapitulates the molecular diversity observed in patients and will serve as useful guides in selecting cell lines for preclinical modeling.

Original language	English (US)
Pages (from-to)	3114-3124
Number of pages	11
Journal	Head and Neck
Volume	41
Issue number	9
DOIs	https://doi.org/10.1002/hed.25803
State	Published - Sep 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cell lines
exome sequencing
HNSCC
laryngeal cancer
precision medicine
UM-SCC

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10.1002/hed.25803

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title = "The molecular landscape of the University of Michigan laryngeal squamous cell carcinoma cell line panel",

abstract = "Background: Laryngeal squamous cell carcinomas (LSCCs) have a high risk of recurrence and poor prognosis. Patient-derived cancer cell lines remain important preclinical models for advancement of new therapeutic strategies, and comprehensive characterization of these models is vital in the precision medicine era. Methods: We performed exome and transcriptome sequencing as well as copy number analysis of a panel of LSCC-derived cell lines that were established at the University of Michigan and are used in laboratories worldwide. Results: We observed a complex array of alterations consistent with those reported in The Cancer Genome Atlas head and neck squamous cell carcinoma project, including aberrations in PIK3CA, EGFR, CDKN2A, TP53, and NOTCH family and FAT1 genes. A detailed analysis of FAT family genes and associated pathways showed disruptions to these genes in most cell lines. Conclusions: The molecular profiles we have generated indicate that as a whole, this panel recapitulates the molecular diversity observed in patients and will serve as useful guides in selecting cell lines for preclinical modeling.",

keywords = "cell lines, exome sequencing, HNSCC, laryngeal cancer, precision medicine, UM-SCC",

author = "Mann, \{Jacqueline E.\} and Aditi Kulkarni and Birkeland, \{Andrew C.\} and Judy Kafelghazal and Julia Eisenberg and Jewell, \{Brittany M.\} and Ludwig, \{Megan L.\} and Spector, \{Matthew E.\} and Hui Jiang and Carey, \{Thomas E.\} and Brenner, \{J. Chad\}",

note = "Publisher Copyright: {\textcopyright} 2019 Wiley Periodicals, Inc.",

year = "2019",

month = sep,

doi = "10.1002/hed.25803",

language = "English (US)",

volume = "41",

pages = "3114--3124",

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T1 - The molecular landscape of the University of Michigan laryngeal squamous cell carcinoma cell line panel

AU - Mann, Jacqueline E.

AU - Kulkarni, Aditi

AU - Birkeland, Andrew C.

AU - Kafelghazal, Judy

AU - Eisenberg, Julia

AU - Jewell, Brittany M.

AU - Ludwig, Megan L.

AU - Spector, Matthew E.

AU - Jiang, Hui

AU - Carey, Thomas E.

AU - Brenner, J. Chad

PY - 2019/9

Y1 - 2019/9

N2 - Background: Laryngeal squamous cell carcinomas (LSCCs) have a high risk of recurrence and poor prognosis. Patient-derived cancer cell lines remain important preclinical models for advancement of new therapeutic strategies, and comprehensive characterization of these models is vital in the precision medicine era. Methods: We performed exome and transcriptome sequencing as well as copy number analysis of a panel of LSCC-derived cell lines that were established at the University of Michigan and are used in laboratories worldwide. Results: We observed a complex array of alterations consistent with those reported in The Cancer Genome Atlas head and neck squamous cell carcinoma project, including aberrations in PIK3CA, EGFR, CDKN2A, TP53, and NOTCH family and FAT1 genes. A detailed analysis of FAT family genes and associated pathways showed disruptions to these genes in most cell lines. Conclusions: The molecular profiles we have generated indicate that as a whole, this panel recapitulates the molecular diversity observed in patients and will serve as useful guides in selecting cell lines for preclinical modeling.

AB - Background: Laryngeal squamous cell carcinomas (LSCCs) have a high risk of recurrence and poor prognosis. Patient-derived cancer cell lines remain important preclinical models for advancement of new therapeutic strategies, and comprehensive characterization of these models is vital in the precision medicine era. Methods: We performed exome and transcriptome sequencing as well as copy number analysis of a panel of LSCC-derived cell lines that were established at the University of Michigan and are used in laboratories worldwide. Results: We observed a complex array of alterations consistent with those reported in The Cancer Genome Atlas head and neck squamous cell carcinoma project, including aberrations in PIK3CA, EGFR, CDKN2A, TP53, and NOTCH family and FAT1 genes. A detailed analysis of FAT family genes and associated pathways showed disruptions to these genes in most cell lines. Conclusions: The molecular profiles we have generated indicate that as a whole, this panel recapitulates the molecular diversity observed in patients and will serve as useful guides in selecting cell lines for preclinical modeling.

KW - cell lines

KW - exome sequencing

KW - HNSCC

KW - laryngeal cancer

KW - precision medicine

KW - UM-SCC

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JO - Head and Neck

JF - Head and Neck

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ER -

The molecular landscape of the University of Michigan laryngeal squamous cell carcinoma cell line panel

Abstract

Bibliographical note

UN SDGs

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