The molecular identities of the caenorhabditis elegans intraflagellar transport genes dyf-6, daf-10 and osm-1

Leslie R. Bell, Steven Stone, John Yochem, Jocelyn E. Shaw, Robert K. Herman

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

The Caenorhabditis elegans genes dyf-6, daf-10, and osm-1 are among the set of genes that affect chemotaxis and the ability of certain sensory neurons to take up fluorescent dyes from the environment. Some genes in this category are known to be required for intraflagellar transport (IFT), which is the bidirectional movement of raft-like particles along the axonemes of cilia and flagella. The cloning of dyf-6, daf-10, and osm-1 are described here. The daf-10 and osm-1 gene products resemble each other and contain WD and WAA repeats. DYF-6, the product of a complex locus, lacks known motifs, but orthologs are present in flies and mammals. Phenotypic analysis of dyf-6 mutants expressing an OSM-6::GFP reporter indicates that the cilia of the amphid and phasmid dendritic endings are foreshortened. Consistent with genetic mosaic analysis, which indicates that dyf-6 functions in neurons of the amphid sensilla, DYF-6::GFP is expressed in amphid and phasmid neurons. Movement of DYF-6::GFP within the ciliated endings of the neurons indicates that DYF-6 is involved in IFT. In addition, IFT can be observed in dauer larvae.

Original languageEnglish (US)
Pages (from-to)1275-1286
Number of pages12
JournalGenetics
Volume173
Issue number3
DOIs
StatePublished - 2006

Fingerprint

Dive into the research topics of 'The molecular identities of the caenorhabditis elegans intraflagellar transport genes dyf-6, daf-10 and osm-1'. Together they form a unique fingerprint.

Cite this