Abstract
The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 984-1006 |
| Number of pages | 23 |
| Journal | Plant Cell |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2017 |
Fingerprint
Cite this
The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing. / Leydon, Alexander R.; Weinreb, Caleb; Venable, Elena; Reinders, Anke; Ward, John M.; Johnson, Mark A.
In: Plant Cell, Vol. 29, No. 5, 05.2017, p. 984-1006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing
AU - Leydon, Alexander R.
AU - Weinreb, Caleb
AU - Venable, Elena
AU - Reinders, Anke
AU - Ward, John M.
AU - Johnson, Mark A.
PY - 2017/5
Y1 - 2017/5
N2 - The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.
AB - The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.
UR - http://www.scopus.com/inward/record.url?scp=85020393835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020393835&partnerID=8YFLogxK
U2 - 10.1105/tpc.16.00816
DO - 10.1105/tpc.16.00816
M3 - Article
C2 - 28400492
AN - SCOPUS:85020393835
VL - 29
SP - 984
EP - 1006
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 5
ER -