The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing

Alexander R. Leydon, Caleb Weinreb, Elena Venable, Anke Reinders, John M. Ward, Mark A. Johnson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.

Original languageEnglish (US)
Pages (from-to)984-1006
Number of pages23
JournalPlant Cell
Volume29
Issue number5
DOIs
StatePublished - May 2017

Fingerprint

Pollen Tube
RNA Sequence Analysis
pollen tubes
Reproduction
Angiospermae
interspecific hybridization
Arabidopsis
germ cells
Arabidopsis thaliana
spermiation
pistil
fertilization (reproduction)
Complex Mixtures
sexual reproduction
Fertilization
Germ Cells
single nucleotide polymorphism
Genes
Plastics
Single Nucleotide Polymorphism

Cite this

The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing. / Leydon, Alexander R.; Weinreb, Caleb; Venable, Elena; Reinders, Anke; Ward, John M.; Johnson, Mark A.

In: Plant Cell, Vol. 29, No. 5, 05.2017, p. 984-1006.

Research output: Contribution to journalArticle

Leydon, Alexander R. ; Weinreb, Caleb ; Venable, Elena ; Reinders, Anke ; Ward, John M. ; Johnson, Mark A. / The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing. In: Plant Cell. 2017 ; Vol. 29, No. 5. pp. 984-1006.
@article{b2b3dfbb24cd40fd8ae6407cab916aa0,
title = "The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing",
abstract = "The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.",
author = "Leydon, {Alexander R.} and Caleb Weinreb and Elena Venable and Anke Reinders and Ward, {John M.} and Johnson, {Mark A.}",
year = "2017",
month = "5",
doi = "10.1105/tpc.16.00816",
language = "English (US)",
volume = "29",
pages = "984--1006",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "5",

}

TY - JOUR

T1 - The molecular dialog between flowering plant reproductive partners defined by snp-informed rna-sequencing

AU - Leydon, Alexander R.

AU - Weinreb, Caleb

AU - Venable, Elena

AU - Reinders, Anke

AU - Ward, John M.

AU - Johnson, Mark A.

PY - 2017/5

Y1 - 2017/5

N2 - The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.

AB - The molecular interactions between reproductive cells are critical for determining whether sexual reproduction between individuals results in fertilization and can result in barriers to interspecific hybridization. However, it is a challenge to define the complete molecular exchange between reproductive partners because parents contribute to a complex mixture of cells during reproduction. We unambiguously defined male- and female-specific patterns of gene expression during Arabidopsis thaliana reproduction using single nucleotide polymorphism-informed RNA-sequencing analysis. Importantly, we defined the repertoire of pollen tube-secreted proteins controlled by a group of MYB transcription factors that are required for sperm release from the pollen tube to the female gametes, a critical barrier to interspecific hybridization. Our work defines the pollen tube gene products that respond to the pistil and are required for reproductive success; moreover, we find that these genes are highly evolutionarily plastic both at the level of coding sequence and expression across A. thaliana accessions.

UR - http://www.scopus.com/inward/record.url?scp=85020393835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020393835&partnerID=8YFLogxK

U2 - 10.1105/tpc.16.00816

DO - 10.1105/tpc.16.00816

M3 - Article

C2 - 28400492

AN - SCOPUS:85020393835

VL - 29

SP - 984

EP - 1006

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 5

ER -