The modulation of tissue factor by endothelial cells during heat shock

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Abstract

Tissue factor (TF) initiates the extrinsic coagulation cascade on the surface of macrophages and endothelial cells. In septic patients, the extrinsic coagulation cascade is activated. When septic patients are febrile, mortality is decreased. The purpose of this study was to investigate the role of elevated temperatures on TF expression by endothelial cells during a sepsis-like challenge. Human endothelial vein cells (HUVECs) were incubated with lipopolysaccharide (LPS) or interleukin-1β (IL-1β) for 0, 2, 4, 6, or 8 h. At the 0-h time point, some HUVECs were heat shocked at 43 °C for 2 h and then recovered at 37 °C for 0, 2, 4, or 6 h. Heat-shocked and non-heat-shocked LPS-stimulated HUVECs were analyzed for TF-specific mRNA expression by ribonuclease protection assay (RPA), surface TF expression by flow cytometry, and TF activity by a two-stage clotting assay. Heat shocked LPS-stimulated HUVECs expressed significantly reduced TF-specific mRNA, TF surface protein levels, and TF surface activity when compared with non-heat-shocked, LPS-stimulated HUVECs (p < 0.0125, p < 0.0125, and p < 0.0001, respectively; repeated measures analysis of variance, ANOVA). If heat shock models elevated core temperature, these results suggest that fever may protect the host during sepsis by reducing TF activity on the surface of endothelial cells.

Original languageEnglish (US)
Pages (from-to)11065-11071
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number13
DOIs
StatePublished - Mar 28 2003

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Endothelial cells
Thromboplastin
Shock
Endothelial Cells
Hot Temperature
Modulation
Veins
Lipopolysaccharides
Analysis of variance (ANOVA)
Coagulation
Assays
Sepsis
Analysis of Variance
Fever
Messenger RNA
Temperature
Flow cytometry
Macrophages
Ribonucleases
Interleukin-1

Cite this

The modulation of tissue factor by endothelial cells during heat shock. / Basi, David L.; Ross, Karen F; Hodges, James S; Herzberg, Mark C.

In: Journal of Biological Chemistry, Vol. 278, No. 13, 28.03.2003, p. 11065-11071.

Research output: Contribution to journalArticle

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