The model polysaccharide potato galactan is actually a mixture of different polysaccharides

Zhangkai Feng, Zhiying Lin, Huazhi Tang, Jie Geng, Yanbo Hu, Kevin H. Mayo, Guihua Tai, Yifa Zhou

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Commercially-supplied potato galactan (PG) is widely used as a model polysaccharide in various bioactivity studies. However, results using this galactan are not always consistent with the stated composition. Here, we assessed its composition by fractionating this commercial PG and purified its primary components: PG-A, PG-B and PG-Cp with weight-averaged molecular weights of 430, 93, and 11.3 kDa, respectively. PG-Cp consists of free β-1,4-galactan chains, whereas PG-A and PG-B are type I rhamnogalacturonans with long β-1,4-galactan side chains of up to 80 Gal residues and short β-1,4-galactan side chains of 0 to 3 Gal residues that display a “trees in lawn” pattern. Structures of these polysaccharides correlate well with their activities in terms of galectin-3 binding and gut bacterial growth assays. Our study clarifies the confusion related to commercial PG, with purified fractions serving as better model polysaccharides in bioactivity investigations.

Original languageEnglish (US)
Article number120889
JournalCarbohydrate Polymers
StatePublished - Aug 1 2023

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (grant numbers 32171276 and 31870796 ), the Scientific and Technologic Foundation of Jilin Province (grant number 20200201004JC ).

Publisher Copyright:
© 2023 Elsevier Ltd


  • Galactan
  • Galectin
  • Microbiota
  • PULs
  • Polysaccharide
  • Polysaccharide utilization
  • RG-I

PubMed: MeSH publication types

  • Journal Article


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