We have previously shown that maintenance of primitive human hematopoietic stem cells is poor when cultured in contact with marrow stromal feeders. However, when separated from stromal contact, human progenitors can be maintained because adhesion mediated proliferation inhibition does not occur. In this study we demonstrate how the murine fetal liver cell line, AFT024, supports primitive human hematopoiesis better in contact cultures compared to primary feeders. We evaluated if better progenitor maintenance in contact with AFT024 cells can be explained by decreased adhesion itself or decreased adhesion mediated inhibition of proliferation. We show that primitive human hematopoietic cells adhered equally well to AFT024 and primary feeders, such as M2-10B4. Further, contact with metabolically inactive AFT024 cells prevented cell cycle progression and decreased maintenance of primitive progenitors to the same extent as contact with M2-10B4 feeders. However, contact with viable AFT024 feeders did not inhibit proliferation, suggesting that AFT024-factors counteract contact mediated inhibition of proliferation. Cytokine production by M2-10B4 and AFT024 cells was similar. Large-size O-sulfated heparan sulfate glycosaminoglycans, known to be important for hematopoietic support, were found only in AFT024-matrix. We hypothesize that these factors may explain, in part, our observations. Finally, we show that more than 100% of primitive myeloid progenitors could be maintained for at least five weeks when cultured in contact with AFT024 feeders in the presence of Interleukin-3 and Macrophage Inflammatory Protein-1α. In conclusion, AFT024 cells produce factor(s), that counteract contact induced growth inhibition of primitive human hematopoietic progenitors, leading to expansion of these cells in contact with the microenvironment.
|Original language||English (US)|
|Number of pages||11|
|Journal||Cell Communication and Adhesion|
|State||Published - 2002|
Bibliographical noteFunding Information:
This work was supported in part by NIH Grants RO1-HL-49930, PO1-CA-65493 and the U.S. Department of Veterans Affairs. We also acknowledge the support of the University of Minnesota Hospitals and Clinics. MP was supported by a Grant from the Deutscher Akademischer Austauschdienst. The authors wish also to thank Brad Anderson, Scott Wissink, and Kirk VanOverbeke for their excellent technical help.
- Human hematopoietic stems cells
- O-sulfated heparane sulfate glycosaminoglycans
- Proliferation inhibition