The methylene chloride fraction of trichosanthis fructus induces apoptosis in U937 cells through the mitochondrial pathway

Eun Ok Lee, Ju Ryoung Lee, Kwan Hyun Kim, Nam In Baek, Soo Jin Lee, Bog Hieu Lee, Kyung Dong Cho, Kyoo Seok Ahn, Sung Hoon Kim

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Trichosanthis kirilowii MAXIM has been used as a folk remedy to treat diabetes, leukemia, and breast cancer. In the present study, the apoptotic mechanism of the methylene chloride fraction of Trichosanthis Fructus (MCTF) was investigated in human leukemic U937 cells. MCTF exhibited antiproliferative effectsagainst U937 cells (IC50=ca. 8 μg/ml). Apoptotic bodies were observed in MCTF-treated U937 cells in the TUNEL assay. We also confirmed that MCTF significantly increases annexin V+/propidium iodide-cells using FACS analysis. MCTF treatment activated caspase-8, -9 and -3, and led to cleaved poly (ADP-ribose) polymerase and release of cytochrome c into cytosol in a concentration-dependent manner, while MCTF did not affect Bax or Bcl-2 protein levels as shown by Western blot analysis. Taken together, these results indicate that MCTF can induce apoptosis in U937 cells chiefly via a mitochondrial-mediated pathway and suggest that Trichosanthis Fructus can be used in cancer treatment as a chemopreventive agent.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Apoptosis
  • Caspase
  • Mitochondrial pathway
  • Trichosanthis kirilowii
  • U937 cell

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